IADR Abstract Archives
Anti-Streptococcal Adhesion of Polydimethylsiloxane / Nano-Silica Composite Superhydrophobic Coating on Enamel Surface
Objectives: To develop a superhydrophobic coating suitable for oral environment and able to resist bacterial adhesion on enamel surface.
Methods: 20 pieces of 5mm * 5mm * 2mm bovine enamel were prepared. Experimental group : Polydimethylsiloxane and nano-silica were dissolved in n-hexane in a certain proportion, ultrasonic dispersion, obtained superhydrophobic coating solution, sprayed on 10 bovine enamel surface.
Control group : 10 bovine enamel surfaces were sprayed with deionized water. The enamel slices of 5 mm * 5 mm * 2 mm were sprayed at 30 cm and 7 kPa in the experimental group and the control group, and dried at 50 °C for 24 h to obtain enamel slices coated with superhydrophobic coating. The wear resistance of coating was tested by water flow impact method ; MMT method was used to detect the effect of the control group and the experimental group on the activity of L929 cells within 4 and 7 days to verify their biocompatibility. The bacterial adhesion of two groups to streptococcus mutans was detected by cfu method. The surface morphology and structure characteristics of the samples were observed by field emission scanning electron microscope, infrared spectrometer and X-ray diffractometer.
Results: The experimental group showed ' raisin bread ' under SEM, and the nano-silica was embedded in PDMS, which was significantly different from the smooth enamel of the control group. The contact angle test results showed that the static contact angles of the experimental group were all greater than 160°, which was significantly greater than that of the control group ( average WAC = 60.22° ) ( P < 0.05 ). The results of cytotoxicity test on 4 and 7 days in the experimental group showed no significant difference from those in the control group ( P < 0.05 ). The results of cfu counting in the control group and the experimental group showed that the anti-adhesion ability of the coating to Streptococcus aerobicus gradually decreased over time, from more than 90% of 3h to 58%.
Conclusions: In this experiment, a superhydrophobic coating with good biocompatibility was successfully constructed on the enamel surface. The coating has good adhesion resistance to Streptococcus mutans, which provides a new idea for oral caries prevention.
Methods: 20 pieces of 5mm * 5mm * 2mm bovine enamel were prepared. Experimental group : Polydimethylsiloxane and nano-silica were dissolved in n-hexane in a certain proportion, ultrasonic dispersion, obtained superhydrophobic coating solution, sprayed on 10 bovine enamel surface.
Control group : 10 bovine enamel surfaces were sprayed with deionized water. The enamel slices of 5 mm * 5 mm * 2 mm were sprayed at 30 cm and 7 kPa in the experimental group and the control group, and dried at 50 °C for 24 h to obtain enamel slices coated with superhydrophobic coating. The wear resistance of coating was tested by water flow impact method ; MMT method was used to detect the effect of the control group and the experimental group on the activity of L929 cells within 4 and 7 days to verify their biocompatibility. The bacterial adhesion of two groups to streptococcus mutans was detected by cfu method. The surface morphology and structure characteristics of the samples were observed by field emission scanning electron microscope, infrared spectrometer and X-ray diffractometer.
Results: The experimental group showed ' raisin bread ' under SEM, and the nano-silica was embedded in PDMS, which was significantly different from the smooth enamel of the control group. The contact angle test results showed that the static contact angles of the experimental group were all greater than 160°, which was significantly greater than that of the control group ( average WAC = 60.22° ) ( P < 0.05 ). The results of cytotoxicity test on 4 and 7 days in the experimental group showed no significant difference from those in the control group ( P < 0.05 ). The results of cfu counting in the control group and the experimental group showed that the anti-adhesion ability of the coating to Streptococcus aerobicus gradually decreased over time, from more than 90% of 3h to 58%.
Conclusions: In this experiment, a superhydrophobic coating with good biocompatibility was successfully constructed on the enamel surface. The coating has good adhesion resistance to Streptococcus mutans, which provides a new idea for oral caries prevention.