IADR Abstract Archives
Soluble Epoxide Hydrolase Inhibitor Promotes Healing of Chemically-Induced Oral Ulcers
Objectives: Oral ulcers are a common disease of oral mucosa, which can arise as a result of complex and diverse causes, such as pressure, trauma, tobacco, oral hygiene habits, diabetes and so on. In many cases, mouth ulcers will disappear within a week or 2 with no treatment, so a conventional treatment is mainly to speed up healing, prevent infection, reduce pain, etc. TPPU (a soluble epoxide hydrolase inhibitor) has been reported to have anti-inflammatory and pro- angiogenesis effects by increasing epoxyeicosatrienoic acids (EETs) levels. However, it is unclear whether TPPU is effective for oral ulcers. Here, we aim to explore a new strategy for treating oral ulcers by targeting EETs metabolism with TPPU.
Methods: Twenty SD rats were used and divided into experimental and control groups. Each group consisted of 10 animals. The chemically-induced oral ulcers were established on the labial mucosa of the mandibular incisors using a round filter paper soaked with 50% glacial acetic acid. After creating an oral ulcer, the ulcer area was treated with a round filter paper soaked with TPPU (10μmol/L) or PBS for 5 minutes daily for three consecutive days in the experimental or control groups, respectively. The healing time and pain threshold of the oral ulcer was measured, and immunohistochemical staining was performed. In vitro, the effects of TPPU on cell proliferation and angiogenesis were tested.
Results: Compared with control group,TPPU treatment reduced the ulcer healing time and increased the pain threshold. Immunohistochemical staining showed reduced inflammatory cell infiltration, more small blood vessels expressing CD31 and VEGF. KI67 and TUNEL staining showed that TPPU promoted cell proliferation and reduced apoptosis. In vitro experiments also showed that TPPU promoted cell proliferation and tube formation.
Conclusions: Our result showed that TPPU accelerates wound healing, prevents infection, alleviate the pain in the ulcer area, which may be related to the ability of TPPU to promote cell proliferation and reduce cells apoptosis, lower levels of inflammation, facilitate angiopoiesis in oral ulcer. Soluble epoxide hydrolase may be a potential therapeutic target for oral ulcers by regulating the metabolism of EETs.
Methods: Twenty SD rats were used and divided into experimental and control groups. Each group consisted of 10 animals. The chemically-induced oral ulcers were established on the labial mucosa of the mandibular incisors using a round filter paper soaked with 50% glacial acetic acid. After creating an oral ulcer, the ulcer area was treated with a round filter paper soaked with TPPU (10μmol/L) or PBS for 5 minutes daily for three consecutive days in the experimental or control groups, respectively. The healing time and pain threshold of the oral ulcer was measured, and immunohistochemical staining was performed. In vitro, the effects of TPPU on cell proliferation and angiogenesis were tested.
Results: Compared with control group,TPPU treatment reduced the ulcer healing time and increased the pain threshold. Immunohistochemical staining showed reduced inflammatory cell infiltration, more small blood vessels expressing CD31 and VEGF. KI67 and TUNEL staining showed that TPPU promoted cell proliferation and reduced apoptosis. In vitro experiments also showed that TPPU promoted cell proliferation and tube formation.
Conclusions: Our result showed that TPPU accelerates wound healing, prevents infection, alleviate the pain in the ulcer area, which may be related to the ability of TPPU to promote cell proliferation and reduce cells apoptosis, lower levels of inflammation, facilitate angiopoiesis in oral ulcer. Soluble epoxide hydrolase may be a potential therapeutic target for oral ulcers by regulating the metabolism of EETs.