IADR Abstract Archives
Effect of 5-FU and β-Cryptoxanthin on Oral Mucosa Derived Keratinocytes
Objectives: Oral mucositis is a common adverse event in chemotherapy, and that reduce patients’ quality of life. β-cryptoxanthin (β-cry) is a provitamin A carotenoid that is contained in citrus fruit. It has been reported that β-cry show anti-oxidant and anti-inflammatory effects. However the effects of β-cry on oral mucositis still remains to be elucidated. 5-fluorouracil (5-FU), an analog of uracil that nonspecifically blocks DNA synthesis, which is often used for head and neck cancer treatment. Thus, in this study, we investigated the effects of 5-FU and β-cry on human oral mucosa derived normal keratinocytes (hOMK).
Methods: hOMK is seeded on culture plate and cultured with 5-FU and/or β-cry. After arbitrary period, cell number, inflammatory cytokines and matrix metalloproteinases (MMPs) mRNA expression and inflammatory cytokines production in hOMK were evaluated. Moreover, we also examined cell number and inflammatory cytokines production of hOMK that was co-stimulated with Porphyromonas gingivalis LPS (P. gingivalis LPS) and 5-FU.
Results: Cell number of hOMK was significantly reduced by 5-FU stimulation. On the other hand, cell number of hOMK was up-regulated by β-cry treatment. mRNA expression of IL-6, IL-8, MMP2 and MMP9 in hOMK, and IL-6 and IL-8 protein production from hOMK were augmented by 5-FU stimulation, while β-cry treatment significantly suppressed IL-8 and MMP9 mRNA expression and IL-8 production provoked by 5-FU stimulation. Co-stimulation of P. gingivalis LPS and 5-FU enhanced IL-6 and IL-8 production from hOMK.
Conclusions: It was revealed that β-cry could enhance cell proliferation, and down-regulate inflammatory cytokine, MMP expression provoked by 5-FU stimulation in hOMK. Moreover, it was indicated that inflammation induced by 5-FU in oral mucosa was exacerbated by P.gingivalis LPS. Taken together, it was suggested that β-cry application may be able to attenuate chemotherapy induced oral mucositis, and concurrent oral hygiene treatment is effective to oral mucosistis.
Methods: hOMK is seeded on culture plate and cultured with 5-FU and/or β-cry. After arbitrary period, cell number, inflammatory cytokines and matrix metalloproteinases (MMPs) mRNA expression and inflammatory cytokines production in hOMK were evaluated. Moreover, we also examined cell number and inflammatory cytokines production of hOMK that was co-stimulated with Porphyromonas gingivalis LPS (P. gingivalis LPS) and 5-FU.
Results: Cell number of hOMK was significantly reduced by 5-FU stimulation. On the other hand, cell number of hOMK was up-regulated by β-cry treatment. mRNA expression of IL-6, IL-8, MMP2 and MMP9 in hOMK, and IL-6 and IL-8 protein production from hOMK were augmented by 5-FU stimulation, while β-cry treatment significantly suppressed IL-8 and MMP9 mRNA expression and IL-8 production provoked by 5-FU stimulation. Co-stimulation of P. gingivalis LPS and 5-FU enhanced IL-6 and IL-8 production from hOMK.
Conclusions: It was revealed that β-cry could enhance cell proliferation, and down-regulate inflammatory cytokine, MMP expression provoked by 5-FU stimulation in hOMK. Moreover, it was indicated that inflammation induced by 5-FU in oral mucosa was exacerbated by P.gingivalis LPS. Taken together, it was suggested that β-cry application may be able to attenuate chemotherapy induced oral mucositis, and concurrent oral hygiene treatment is effective to oral mucosistis.