IADR Abstract Archives
Apolipoprotein E Regulates Titanium Implant Osseointegration via Osteogenesis-Lipogenesis Balance
Objectives: Apolipoprotein E (ApoE) plays a critical role in the process of bone metabolism. However, the impact of ApoE on implant osseointegration has not yet been clarified. Therefore, investigating the association of ApoE and osseointegration may provide new insight into the osseointegration process. This study aims to investigate the influence of ApoE deficiency and exogenous supplementation of APOE on implant osseointegration in ApoE-/- mice, as well as whether the osteogenesis-adipogenesis balance is involved in this process.
Methods: Animals were divided to the following groups: ApoE+/+ mice, ApoE-/- mice, and ApoE-/- mice with exogenous supplementation of APOE. The expression of APOE was detected by immunohistochemistry staining. The osseointegration of implant was detected by Micro-CT and undecalcified sections staining. The level of osteogenic-related markers (osteocalcin and runt-related transcription factor 2) and adipogenic-related markers (fatty acid binding protein 4 and peroxisome proliferator-activated receptor γ) were detected by immunofluorescence staining.
Results: The results of immunohistochemistry demonstrated the successful exogenous supplementation of APOE in ApoE-/- mice around the experimental implant. Micro-CT and undecalcified sections staining indicated an increase of bone volume/total volume (BV/TV) and bone-implant contact (BIC), as well as a decrease of the adipocyte area proportion around the implant after 4-week healing in the APOE supplementary group compared with the ApoE-/- group. Moreover, the level of osteogenic-related markers elevated and the level of adipogenic-related markers decreased in the APOE supplementary group, compared to untreated ApoE-/- mice.
Conclusions: In summary, ApoE plays a significant role in the process of implant osseointegration by regulation of osteogenesis-lipogenesis balance, which reveals the underlying mechanism of implant osseointegration and provides a potential method to promote implant osseointegration in the future.
Methods: Animals were divided to the following groups: ApoE+/+ mice, ApoE-/- mice, and ApoE-/- mice with exogenous supplementation of APOE. The expression of APOE was detected by immunohistochemistry staining. The osseointegration of implant was detected by Micro-CT and undecalcified sections staining. The level of osteogenic-related markers (osteocalcin and runt-related transcription factor 2) and adipogenic-related markers (fatty acid binding protein 4 and peroxisome proliferator-activated receptor γ) were detected by immunofluorescence staining.
Results: The results of immunohistochemistry demonstrated the successful exogenous supplementation of APOE in ApoE-/- mice around the experimental implant. Micro-CT and undecalcified sections staining indicated an increase of bone volume/total volume (BV/TV) and bone-implant contact (BIC), as well as a decrease of the adipocyte area proportion around the implant after 4-week healing in the APOE supplementary group compared with the ApoE-/- group. Moreover, the level of osteogenic-related markers elevated and the level of adipogenic-related markers decreased in the APOE supplementary group, compared to untreated ApoE-/- mice.
Conclusions: In summary, ApoE plays a significant role in the process of implant osseointegration by regulation of osteogenesis-lipogenesis balance, which reveals the underlying mechanism of implant osseointegration and provides a potential method to promote implant osseointegration in the future.