IADR Abstract Archives

Comparison of Disinfection Performance Between LED and Conventional UVGIs

Objectives: Ultraviolet (UV) light is one of the technologies widely used for inactivating bacteria, viruses, and fungi in disinfection of surfaces. UVC irradiation (200-280 nm) is the main source of the germicidal effect. The present study was to evaluate the disinfection performance of LED and mercury lamps based conventional UV germicidal irradiation (UVGI).
Methods: Porphyromonas gingivalis was spread on the TYHK-agar plates. Plates were covered with plastic lids covered with tin foil to block UV light. A square section is cut from the foil to create a window for controlled exposure of the agar to UV light. Then the plates were treated by the following three commercial portable UVGI sanitizers at the wavelength of 253.7 nm for 1s, 5s, and 10s respectively (Table 1). No UVC treatment and Ampicllin were used as negative and positive controls, respectively. All the plates were then incubated in an anaerobic condition for 48h. Experiment was performed in triplicate to minimize the errors.
Results: Figure 1 presents a typical experimental progression through time. Brand A could kill P. gingivalis after 5s treatment, while Brand B needed 10s. Brand C with LED lamps could kill all the P. gingivalis after almost 1s.
Conclusions: LED UVGI is much more effective than the mercury lamps based conventional UVGI, probably due to higher power and output UV intensity.
Division:
Meeting: 2021 IADR/AADR/CADR General Session (Virtual Experience)
Location:
Year: 2021
Final Presentation ID: 1002
Abstract Category|Abstract Category(s): Microbiology/Immunology
Authors
  • Huang, Xuelian  ( University of Washington , Seattle , Washington , United States )
  • Xu, Kevin  ( School of Dentistry, University of Washington , Seattle , Washington , United States )
  • Jain, Sumita  ( University of Washington , Seattle , Washington , United States )
  • Chan, Daniel  ( University of Washington , Seattle , Washington , United States )
  • Financial Interest Disclosure: None
    SESSION INFORMATION
    Poster Session
    Antimicrobial Strategies, Properties & Therapies II
    Thursday, 07/22/2021 , 11:00AM - 12:00PM
    TABLES
    Table 1. Three commercial portable UVGI sanitizers
    BrandLampPower (W)Intensity (mW/cm2)
    AMercury43
    BMercury52.5
    CLED8065

    IMAGES