IADR Abstract Archives
Antiviral Activity of CloSYS® Oral Care Products Against Influenza a
Objectives: To determine the antiviral activity of stabilized chlorine dioxide containing CloSYS® oral care products towards Influenza A.
Methods: Influenza A virus (H3N2), A/Hong Kong/8/68 was propagated in MDCK cells (ATCC CCL-34). 2.7 mL of test product was mixed with 0.3 mL of test virus and incubated for 30 and 60 seconds. 3.0 mL of 0.5% sodium thiosulfate (neutralizer) was then added, mixed and serially diluted in dilution medium in a 96 well plate. Appropriate virus, neutralization effectiveness/viral interference, cytotoxicity, cell viability, and media sterility controls were run. All plates were incubated in a CO2 incubator for 7 days at the appropriate temperature for the virus. Cytopathic/cytotoxic effects were monitored using an Inverted Compound Microscope. The 50% Tissue Culture Infectious Dose per mL (TCID50/mL) was determined by using standard methods.
Results: CloSYS® Ultra Sensitive rinse, Sensitive rinse, Oral spray and Sensitive fluoride toothpaste reduced the initial viral load of Influenza A H3N2 by 99.993%, 99.9% to 99.982%, 99.944% to 99.968% and 99.766% within 30 seconds of contact time, respectively. There did not appear to be a substantive difference in the antiviral activity observed at 30 and 60 seconds of contact time. The log10 reduction values at 60 seconds contact time were 4.38 to 4.63, 4.0, 3.5 to 4.0, and 2.88 to 3.13 for the Ultra Sensitive rinse, Sensitive rinse, Oral spray and Sensitive fluoride toothpaste, respectively, demonstrating their strong antiviral activity against Influenza A H3N2. Slightly lower log10 reduction by Sensitive fluoride toothpaste relative to other products is attributed to use of 1à3 diluted toothpaste slurry. The results are important since comorbidity of Influenza A and pneumonia with COVID-19 results in clinical complications.
Conclusions: These in vitro studies demonstrate that CloSYS® Ultra Sensitive rinse, Sensitive rinse, Oral spray and Sensitive fluoride toothpaste exhibit strong antiviral activity against Influenza A H3N2.
Methods: Influenza A virus (H3N2), A/Hong Kong/8/68 was propagated in MDCK cells (ATCC CCL-34). 2.7 mL of test product was mixed with 0.3 mL of test virus and incubated for 30 and 60 seconds. 3.0 mL of 0.5% sodium thiosulfate (neutralizer) was then added, mixed and serially diluted in dilution medium in a 96 well plate. Appropriate virus, neutralization effectiveness/viral interference, cytotoxicity, cell viability, and media sterility controls were run. All plates were incubated in a CO2 incubator for 7 days at the appropriate temperature for the virus. Cytopathic/cytotoxic effects were monitored using an Inverted Compound Microscope. The 50% Tissue Culture Infectious Dose per mL (TCID50/mL) was determined by using standard methods.
Results: CloSYS® Ultra Sensitive rinse, Sensitive rinse, Oral spray and Sensitive fluoride toothpaste reduced the initial viral load of Influenza A H3N2 by 99.993%, 99.9% to 99.982%, 99.944% to 99.968% and 99.766% within 30 seconds of contact time, respectively. There did not appear to be a substantive difference in the antiviral activity observed at 30 and 60 seconds of contact time. The log10 reduction values at 60 seconds contact time were 4.38 to 4.63, 4.0, 3.5 to 4.0, and 2.88 to 3.13 for the Ultra Sensitive rinse, Sensitive rinse, Oral spray and Sensitive fluoride toothpaste, respectively, demonstrating their strong antiviral activity against Influenza A H3N2. Slightly lower log10 reduction by Sensitive fluoride toothpaste relative to other products is attributed to use of 1à3 diluted toothpaste slurry. The results are important since comorbidity of Influenza A and pneumonia with COVID-19 results in clinical complications.
Conclusions: These in vitro studies demonstrate that CloSYS® Ultra Sensitive rinse, Sensitive rinse, Oral spray and Sensitive fluoride toothpaste exhibit strong antiviral activity against Influenza A H3N2.