IADR Abstract Archives
Stress Alters Diverse Dental MSCs Differentiation – Pre-implant Clinical Consideration
Objectives: Functional capabilities of stem cells of dental origin have been known to be
influenced by smoking (induced stress) and metabolic stress (diabetes). In
the current investigation we studied the impact of these induced and
metabolic stress with respect to pre-impact consideration on differentiation
and bone formation.
Methods: Progenitor stem cells isolated from dental pulp, follicle and gingival tissues
were phenotypes and subjected to nicotine and high glucose stress
mimicking smoking and diabetic condition in-vitro. The specific gene
transcriptional markers pertaining to survival and differentiation to
osteoblast and cytokine signaling pertinent of the modulatory functions
were assessed and correlated with phenotypic differentiation.
Results: The cells isolated were initially confirmed as stem cells by assessing the
expression of Endoglin and alanyl aminopeptidase for dental pulp stem cells
(DPSCs), BCRP for follicular stem cells (GFSCs) and stro-1 and Oct-4 for
gingival stromal cells (GMSCs). These cells were then subjected to 100uM
Nicotine treatment and 10uM glucose individually for 24 hours. The
cellular viability post treatment was about 86% to 89% in both the
treatments respectively while about 73% viability was observed in
combined nicotine and glucose treatment. Further, we observed a
comparative expression of survival genes at mRNA levels in all the three
cell types. The expression of osteoblast differentiation was varied with
similar expression in DPSCs and GMSCs while GFSC showed very
minimal differentiation. We observed elevated levels of pro inflammatory
cytokines in all the three cell groups substantiating the cell survival further
augment cell proliferation. The other two sources were characterized with
anti-inflammatory or paracrine cytokines akin to their differentiation gene markers.
Conclusions: From the current study, it is evident that the stem cells of varied dental
origin responded to the stress are more or less uniform with physiological
delay in differentiation into osteoblast. Further, their modulatory function
was dependent on their type to be either regenerative or modulatory in
functions.
influenced by smoking (induced stress) and metabolic stress (diabetes). In
the current investigation we studied the impact of these induced and
metabolic stress with respect to pre-impact consideration on differentiation
and bone formation.
Methods: Progenitor stem cells isolated from dental pulp, follicle and gingival tissues
were phenotypes and subjected to nicotine and high glucose stress
mimicking smoking and diabetic condition in-vitro. The specific gene
transcriptional markers pertaining to survival and differentiation to
osteoblast and cytokine signaling pertinent of the modulatory functions
were assessed and correlated with phenotypic differentiation.
Results: The cells isolated were initially confirmed as stem cells by assessing the
expression of Endoglin and alanyl aminopeptidase for dental pulp stem cells
(DPSCs), BCRP for follicular stem cells (GFSCs) and stro-1 and Oct-4 for
gingival stromal cells (GMSCs). These cells were then subjected to 100uM
Nicotine treatment and 10uM glucose individually for 24 hours. The
cellular viability post treatment was about 86% to 89% in both the
treatments respectively while about 73% viability was observed in
combined nicotine and glucose treatment. Further, we observed a
comparative expression of survival genes at mRNA levels in all the three
cell types. The expression of osteoblast differentiation was varied with
similar expression in DPSCs and GMSCs while GFSC showed very
minimal differentiation. We observed elevated levels of pro inflammatory
cytokines in all the three cell groups substantiating the cell survival further
augment cell proliferation. The other two sources were characterized with
anti-inflammatory or paracrine cytokines akin to their differentiation gene markers.
Conclusions: From the current study, it is evident that the stem cells of varied dental
origin responded to the stress are more or less uniform with physiological
delay in differentiation into osteoblast. Further, their modulatory function
was dependent on their type to be either regenerative or modulatory in
functions.
