IADR Abstract Archives
Establishment of a Three-Dimensional Hydroxyapatite Scaffold for Alveolar Ridge Regeneration
Objectives: The aim of this study was to develop a rapidly protyped hydroxyapatite scaffold to promote the regeneration of large-sized intrabony defects and supra-alveolar augmentation.
Methods: A hydroxyapatite-based scaffold with orthogonal pores was designed. The scaffold was prepared by a bioprinter using a bio-ink composed of 90% hydroxyapatite and 10% bio-derived polymer (polylactic-co-glycolic acid, PLGA) and was printed at linear deposition rates of 1-2 mm/s using 22G nozzles with an extrusion-based additive manufacturing, direct-ink-writing technique. The preclinical feasibility of the scaffold was assessed by implanting these scaffolds to the surgically created intrabony defects (4x4 mm, through-and-through) and the area of supra-alveolar ridge augmentation (5x5 mm, with 2.5 mm thickness) on the mandibles of Sprague-Dawley rats for 4 weeks, and the treatment outcome was assessed by micro-CT imaging and histology.
Results: The internal microstructures of the hydroxyapatite scaffold were homogenously porous and theoretical pore size was 300-400 μm. With 148 kPa of printing pressure, the pore size was 0.42±0.028 mm by 0.328±0.005 mm, and the thickness of struct was 0.449±0.101 mm. At 4 weeks, micro-CT imaging revealed that the intrabony defect was rapid resolved with the treatment of hydroxyapatite scaffold and revealed the evidences that new bone grew to and entrapped in the supra-alveolar scaffold. The integration of newly-formed bone and scaffold was also evident from histology.
Conclusions: The rapid-prototyped hydroxyapatite scaffold showed acceptable geometrical stability and promising osteoconductive properties in vivo. By further justification, the scaffold can be a potential alternative to current bone grafting materials.
Methods: A hydroxyapatite-based scaffold with orthogonal pores was designed. The scaffold was prepared by a bioprinter using a bio-ink composed of 90% hydroxyapatite and 10% bio-derived polymer (polylactic-co-glycolic acid, PLGA) and was printed at linear deposition rates of 1-2 mm/s using 22G nozzles with an extrusion-based additive manufacturing, direct-ink-writing technique. The preclinical feasibility of the scaffold was assessed by implanting these scaffolds to the surgically created intrabony defects (4x4 mm, through-and-through) and the area of supra-alveolar ridge augmentation (5x5 mm, with 2.5 mm thickness) on the mandibles of Sprague-Dawley rats for 4 weeks, and the treatment outcome was assessed by micro-CT imaging and histology.
Results: The internal microstructures of the hydroxyapatite scaffold were homogenously porous and theoretical pore size was 300-400 μm. With 148 kPa of printing pressure, the pore size was 0.42±0.028 mm by 0.328±0.005 mm, and the thickness of struct was 0.449±0.101 mm. At 4 weeks, micro-CT imaging revealed that the intrabony defect was rapid resolved with the treatment of hydroxyapatite scaffold and revealed the evidences that new bone grew to and entrapped in the supra-alveolar scaffold. The integration of newly-formed bone and scaffold was also evident from histology.
Conclusions: The rapid-prototyped hydroxyapatite scaffold showed acceptable geometrical stability and promising osteoconductive properties in vivo. By further justification, the scaffold can be a potential alternative to current bone grafting materials.
