HPDLSC Viability and Differentiation Potential in Avulsed Tooth Storage Media

Objectives: Immediate replantation has been recommended as the best method of management of tooth avulsion
by IADT. In event of a delay, tooth needs to be preserved in appropriate storage medium. Studies have evaluated Fibroblast viability in large number of storage media. However these cells rarely function in seclusion. It has been hypothesized that
human Periodontal Ligament Stem Cells(hPDLSC) have major role in success of replantation. Present study was planned to assess the effect of various storage media on viability, proliferative ability and differentiation potential of hPDLSCs.
Methods: hPDLSCs were isolated and characterized from mature permanent teeth extracted atraumatically from healthy human subjects of 14-24 years for orthodontic purposes. Media tested were: a. Hanks Balanced Salt Solution b. Aloe Vera c. Egg White d. HBSS + Egg White and f. Oral Rehydration Solution. Images were documented; cell viability was assessed using MTT assay and their differentiation potential was evaluated.
Results: HBSS + egg white (87.2±8.6) had highest percent cell viability and differentiation potential followed by ORS-L and egg white at 150 min. HBSS + egg white had highest percent cell viability (30.3±2.9) at 96 hrs followed by ORS-L, egg white, HBSS and aloe vera(1.3±0.6).
Conclusions: The combination of Egg White + HBSS was found to be the most effective storage media for hPDLSCs followed by ORS, HBSS, Egg
White and Aloe Vera (10%).