IADR Abstract Archives
PLGA/LDH Scaffolds Through Microsphere Sintering Method For Maxillofacial Bone Regeneration
Objectives: The aim of this study was to develop novel 3D scaffolds made of poly(lactic-co-glycolic acid) (PLGA)/layered double hydroxide (LDH) composite for maxillofacial bone regeneration applications.
Methods: The 3D PLGA/LDH microsphere-sintered composite scaffolds with various ratios were produced through microsphere sintering method. The pure PLGA and PLGA microspheres including LDH (mean particle size of 70 nm) as a secondary phase with PLGA/LDH ratio (1:1), (10:1) and (4:1) were packed tightly in a stainless mold and sintered above the glass transition temperature of PLGA for a certain time. Different sintering temperatures (80 or 90 C) and sintering times (2, 3 and 4 h) on the mechanical properties of the scaffolds were evaluated. Also, the structural, and biological properties of the scaffolds were investigated.
Results: The obtained results ascertained that the PLGA/LDH (4:1) scaffold sintered at 90 C for 2h had the highest mechanical characteristics and also had an appropriate pore structure for maxillofacial bone regeneration applications and selected as an optimum scaffold. The MTT assay results showed that the cell proliferation on the composite optimum scaffolds was higher than pure PLGA scaffold and control group. Osteoblasts cultured on the optimum composite scaffolds, had higher alkaline phosphatase activity (ALP activity) than those cultured on the pure PLGA scaffold and control group.
Conclusions: The 3D PLGA/LDH microsphere-sintered composite scaffold may have future implications as a scaffold for maxillofacial bone regeneration applications.
Methods: The 3D PLGA/LDH microsphere-sintered composite scaffolds with various ratios were produced through microsphere sintering method. The pure PLGA and PLGA microspheres including LDH (mean particle size of 70 nm) as a secondary phase with PLGA/LDH ratio (1:1), (10:1) and (4:1) were packed tightly in a stainless mold and sintered above the glass transition temperature of PLGA for a certain time. Different sintering temperatures (80 or 90 C) and sintering times (2, 3 and 4 h) on the mechanical properties of the scaffolds were evaluated. Also, the structural, and biological properties of the scaffolds were investigated.
Results: The obtained results ascertained that the PLGA/LDH (4:1) scaffold sintered at 90 C for 2h had the highest mechanical characteristics and also had an appropriate pore structure for maxillofacial bone regeneration applications and selected as an optimum scaffold. The MTT assay results showed that the cell proliferation on the composite optimum scaffolds was higher than pure PLGA scaffold and control group. Osteoblasts cultured on the optimum composite scaffolds, had higher alkaline phosphatase activity (ALP activity) than those cultured on the pure PLGA scaffold and control group.
Conclusions: The 3D PLGA/LDH microsphere-sintered composite scaffold may have future implications as a scaffold for maxillofacial bone regeneration applications.