IADR Abstract Archives
Release and Recharge of Fluoride, Calcium and Strontium Releasing Materials
Objectives: A series of new restorative materials that release fluoride and calcium have been developed at GC America and other companies. The purpose of this study was to assess the ability of these materials to take up fluoride and calcium ions when exposed to MI Paste-ONE and release these ions later.
Methods: Ten pellets were made from each of six products [GC Fuji Automix LC, ‘FAM’; GC EQUIA Forte coated, ‘EQF-EFC’; GC EQUIA Forte no-coat, ‘EQF’; GC Fuji Triage, ‘FTR’; Pulpdent Activa ‘ABA’; Shofu BEAUTIFIL II, ‘BF2’]. Five pellets of each type were depleted of Ca and F by immersion in 3.5mL of either lactic acid (pH 4.6, 50mmol/L) or HEPES buffer (pH7.0 50mmol/L) and agitated on an orbital shaker. On days 1, 2, 7, 14, 21 and 30 aliquots were collected for Ca, Sr, F and pH analyses and the samples placed in fresh lactic acid or HEPES solutions as appropriate. After aliquot collection, on day 30, a slurry of MI Paste-ONE and H2O (1:2) was used to recharge the samples. The MI Paste-ONE/H2O recharge was done twice on day 30 and once a on days 31, 32 and 37. Aliquots (1mL) from each vial were collected on these days for pH, F, Ca and Sr analyses. pH and F were analyzed via ion-selective electrode on the day of collection. Ca and Sr were acidified in HNO3 and analyzed via ICP-MS later.
Results: See the chart for the cumulative release of F, Ca and Sr at pH 4.6 and 7.0. The release of F, Ca and Sr was significantly higher at acidic pH than at neutral pH for FTR and EQF (p<0.01). The MI Paste-ONE recharges after day 30 caused an increase in the release rate for EQF and FTR for F and Ca at acidic pH.
Conclusions: GC Equia Forte and GC Fuji Triage contain soluble F and Ca mineral that is released in significant amounts at acidic pH. These materials also absorb and release F and Ca from MI Paste-ONE. These F and Ca materials may have potential for reducing secondary demineralization.
Methods: Ten pellets were made from each of six products [GC Fuji Automix LC, ‘FAM’; GC EQUIA Forte coated, ‘EQF-EFC’; GC EQUIA Forte no-coat, ‘EQF’; GC Fuji Triage, ‘FTR’; Pulpdent Activa ‘ABA’; Shofu BEAUTIFIL II, ‘BF2’]. Five pellets of each type were depleted of Ca and F by immersion in 3.5mL of either lactic acid (pH 4.6, 50mmol/L) or HEPES buffer (pH7.0 50mmol/L) and agitated on an orbital shaker. On days 1, 2, 7, 14, 21 and 30 aliquots were collected for Ca, Sr, F and pH analyses and the samples placed in fresh lactic acid or HEPES solutions as appropriate. After aliquot collection, on day 30, a slurry of MI Paste-ONE and H2O (1:2) was used to recharge the samples. The MI Paste-ONE/H2O recharge was done twice on day 30 and once a on days 31, 32 and 37. Aliquots (1mL) from each vial were collected on these days for pH, F, Ca and Sr analyses. pH and F were analyzed via ion-selective electrode on the day of collection. Ca and Sr were acidified in HNO3 and analyzed via ICP-MS later.
Results: See the chart for the cumulative release of F, Ca and Sr at pH 4.6 and 7.0. The release of F, Ca and Sr was significantly higher at acidic pH than at neutral pH for FTR and EQF (p<0.01). The MI Paste-ONE recharges after day 30 caused an increase in the release rate for EQF and FTR for F and Ca at acidic pH.
Conclusions: GC Equia Forte and GC Fuji Triage contain soluble F and Ca mineral that is released in significant amounts at acidic pH. These materials also absorb and release F and Ca from MI Paste-ONE. These F and Ca materials may have potential for reducing secondary demineralization.
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