IADR Abstract Archives
Salivary Proteomic Biomarkers For Non-Invasive Diagnosis Of Breast Cancer
Objectives: Breast cancer is the second most common cancer in the world and the leading cause of cancer death in the Brazilian female population. Its early detection is of paramount importance to improve the patient's survival rate. Saliva has been recognized as a promising diagnostic method for detecting diseases, including cancer. Thus, this study proposes to identify biomarkers for the early diagnosis of breast cancer through the analysis of the salivary proteome of transgenic mice that develop endogenous breast cancer.
Methods: The C(3)1-Tag is a genetically modified mice model that develops endogenous breast cancer by inhibiting tumor suppressor genes such as p53. Saliva was collected at 4, 8, 20, 24 and 28 weeks of age from seven C(3)1-Tag mice and 7 negative littermates females. Salivation was induced by subcutaneous injection of pilocarpine; saliva was obtained from the oral cavity by micropipette. The samples were stored in -80 °C until analyzed. Proteomic analysis was performed by label- free mass spectrometry. Results were computed to assess and correlate protein patterns of disease and control mice.
Results: Our preliminary results show that mice with 4 and 8 weeks of age that do not show cellular alterations indicative of lesion in mammary histopathology, already have alterations in protein expression in saliva. These animals have a higher protein expression of the pathways related to inflammation, angiogenesis and hypoxia. Animals of these ages that do not have genetic alteration to develop breast cancer have the protein expression in saliva most related to the pentose phosphate pathway, which is naturally expressed in adipose tissue richly present in breast, and blood coagulation important for homeostasis.
Conclusions: Our results show that protein analyzes of saliva may be promising data to identify cellular changes present in tumor cells that are not yet detected by the classical histopathology method.
Methods: The C(3)1-Tag is a genetically modified mice model that develops endogenous breast cancer by inhibiting tumor suppressor genes such as p53. Saliva was collected at 4, 8, 20, 24 and 28 weeks of age from seven C(3)1-Tag mice and 7 negative littermates females. Salivation was induced by subcutaneous injection of pilocarpine; saliva was obtained from the oral cavity by micropipette. The samples were stored in -80 °C until analyzed. Proteomic analysis was performed by label- free mass spectrometry. Results were computed to assess and correlate protein patterns of disease and control mice.
Results: Our preliminary results show that mice with 4 and 8 weeks of age that do not show cellular alterations indicative of lesion in mammary histopathology, already have alterations in protein expression in saliva. These animals have a higher protein expression of the pathways related to inflammation, angiogenesis and hypoxia. Animals of these ages that do not have genetic alteration to develop breast cancer have the protein expression in saliva most related to the pentose phosphate pathway, which is naturally expressed in adipose tissue richly present in breast, and blood coagulation important for homeostasis.
Conclusions: Our results show that protein analyzes of saliva may be promising data to identify cellular changes present in tumor cells that are not yet detected by the classical histopathology method.