IADR Abstract Archives
Bioactivity, Osteogenic and Angiogenic Potential of Sodium-free Fluoride-containing Bioactive Glasses
Objectives: To study the bioactivity of sodium-free fluoride-containing BGs (FBGs) in α-MEM and discover the in vitro osteogenic and angiogenic potential of FBGs-conditioned α-MEM on mouse osteoblast-like cell line MC3T3-E1.
Methods: A series of FBGs (SiO2-P2O5-CaO-CaF2, 0-9.3 mol% CaF2) was synthesized by a melt-quench route. The ion release profiles (Calcium, Phosphorus and Fluoride) and apatite formation of FBGs in α-MEM were explored by ICP-OES, fluoride-ion selective electrode and FTIR. The cytotoxicity of culture media containing ions released from FBGs was evaluated by MTT assay. Alkaline phosphatase (ALP) activity in MC3T3-E1 cultured with BG-conditioned media was assessed by enzyme histochemical assay. The mineralization and collagen formation abilities of FBGs were quantified by Alizarin Red S staining and Sirius Red staining. The expression of osteogenic and angiogenic genes in MC3T3-E1 was investigated by qRT-PCR.
Results: Rapid ion release and apatite formation in α-MEM were observed for all FBGs. Characteristic FTIR apatite bands were noticed for FBGs after 9 h immersion. Cell viability suggested that the studied FBGs were non-cytotoxic. Lower ALP activity was found for FBGs with higher fluoride contents. The addition of fluoride promoted mineralization, collagen formation and the expression of osteogenic and angiogenic genes in MC3T3-E1. Compared to fluoride-containing BGs with sodium (NaFBGs), a higher amount of fluoride was incorporated into FBGs resulted in improved glass biological effects.
Conclusions: The bioactivity of FBGs and their osteogenic and angiogenic potential on osteoblasts-like MC3T3-E1 in α-MEM were investigated for the first time. These highly bioactive FBGs were non-cytotoxic and offered distinct angiogenic and osteogenic response to MC3T3-E1 suggesting that they could be safe and attractive for clinical application as bone substitutes.
Methods: A series of FBGs (SiO2-P2O5-CaO-CaF2, 0-9.3 mol% CaF2) was synthesized by a melt-quench route. The ion release profiles (Calcium, Phosphorus and Fluoride) and apatite formation of FBGs in α-MEM were explored by ICP-OES, fluoride-ion selective electrode and FTIR. The cytotoxicity of culture media containing ions released from FBGs was evaluated by MTT assay. Alkaline phosphatase (ALP) activity in MC3T3-E1 cultured with BG-conditioned media was assessed by enzyme histochemical assay. The mineralization and collagen formation abilities of FBGs were quantified by Alizarin Red S staining and Sirius Red staining. The expression of osteogenic and angiogenic genes in MC3T3-E1 was investigated by qRT-PCR.
Results: Rapid ion release and apatite formation in α-MEM were observed for all FBGs. Characteristic FTIR apatite bands were noticed for FBGs after 9 h immersion. Cell viability suggested that the studied FBGs were non-cytotoxic. Lower ALP activity was found for FBGs with higher fluoride contents. The addition of fluoride promoted mineralization, collagen formation and the expression of osteogenic and angiogenic genes in MC3T3-E1. Compared to fluoride-containing BGs with sodium (NaFBGs), a higher amount of fluoride was incorporated into FBGs resulted in improved glass biological effects.
Conclusions: The bioactivity of FBGs and their osteogenic and angiogenic potential on osteoblasts-like MC3T3-E1 in α-MEM were investigated for the first time. These highly bioactive FBGs were non-cytotoxic and offered distinct angiogenic and osteogenic response to MC3T3-E1 suggesting that they could be safe and attractive for clinical application as bone substitutes.
