IADR Abstract Archives
Adjunctive 445nm Laser Irradiation for Root Canal Disinfection
Objectives: Objectives: The success of root canal treatment depends on a sufficient elimination of pathogenic germs. The aim of the present study was to evaluate a novel 445 nm semiconductor laser for its germ-reducing effect during chemomechanical root canal treatment.
Methods: Methods: As part of the regular endodontic treatment regimen, microbiological specimens of 57 patients were collected with paper points after endodontic emergency treatment in the following sequence: (I) removal of the temporary filling material, (II) chemomechanical treatment (hand/machine-driven instrumentation (ProTaper Gold, Dentsply), rinsing with sodium hypochlorite (3%), (III) according to one of three adjuvant group-specific protocols. As adjuvant protocols (n=19 each) following groups were defined (ethics committee approval: ref.no.016/1749): (a) sodium hypochlorite rinsing (3%), (b) laser irradiation, (c) combination of sodium hypochlorite rinsing and laser irradiation. The diode laser (SiroLaser Blue, Dentsply Sirona, Bensheim) in groups (b) and (c) was used with the settings 0.6W in continuous wave mode (cw) for 4x10s. The samples were collected after flooding the root canal with saline solution, transferred into transport vessels and analyzed microbiologically by quantitative real-time PCR: Total bacterial load (TBL) was assessed as main parameter. Statistical analysis was performed with non-parametric tests (Wilcoxon, Mann-Whitney).
Results: Results: In all three groups, a statistically significant reduction could be observed (p<0.05). The percentual reduction of bacteria was: sole laser therapy 58.2% (min: 27.5, max: 99.9), sole sodium hypochlorite rinsing 80.5% (min: 61.4; max: 99.4). Both groups showed less reduction than the combination of sodium hypochlorite rinsing and 445 nm laser irradiation with 92.7% (min: 61.4, max: 99.7) (p>0.05).
Conclusions:
Conclusion: The present study showed the statistically significant best bacterial reduction with combined 445nm laser irradiation and sodium hypochlorite rinsing. Thus, adjunctive 445nm laser irradiation might improve the clinical outcome of endodontically treated teeth.
Methods: Methods: As part of the regular endodontic treatment regimen, microbiological specimens of 57 patients were collected with paper points after endodontic emergency treatment in the following sequence: (I) removal of the temporary filling material, (II) chemomechanical treatment (hand/machine-driven instrumentation (ProTaper Gold, Dentsply), rinsing with sodium hypochlorite (3%), (III) according to one of three adjuvant group-specific protocols. As adjuvant protocols (n=19 each) following groups were defined (ethics committee approval: ref.no.016/1749): (a) sodium hypochlorite rinsing (3%), (b) laser irradiation, (c) combination of sodium hypochlorite rinsing and laser irradiation. The diode laser (SiroLaser Blue, Dentsply Sirona, Bensheim) in groups (b) and (c) was used with the settings 0.6W in continuous wave mode (cw) for 4x10s. The samples were collected after flooding the root canal with saline solution, transferred into transport vessels and analyzed microbiologically by quantitative real-time PCR: Total bacterial load (TBL) was assessed as main parameter. Statistical analysis was performed with non-parametric tests (Wilcoxon, Mann-Whitney).
Results: Results: In all three groups, a statistically significant reduction could be observed (p<0.05). The percentual reduction of bacteria was: sole laser therapy 58.2% (min: 27.5, max: 99.9), sole sodium hypochlorite rinsing 80.5% (min: 61.4; max: 99.4). Both groups showed less reduction than the combination of sodium hypochlorite rinsing and 445 nm laser irradiation with 92.7% (min: 61.4, max: 99.7) (p>0.05).
Conclusions:
Conclusion: The present study showed the statistically significant best bacterial reduction with combined 445nm laser irradiation and sodium hypochlorite rinsing. Thus, adjunctive 445nm laser irradiation might improve the clinical outcome of endodontically treated teeth.