Anti-Ro/SSA and Antinuclear Antibodies Among Sjögren’s and non-Sjögren’s

Objectives: Detection of ANA and anti-Ro/SSA is essential in diagnosing Sjögren’s syndrome. The objective of the study is to detect Anti-Ro/SSA and difference between antinuclear antibodies pattern in Sjögren’s and non-Sjögren’s patients by Sjo system.
Methods: 43 patients with (N=21 previously diagnosed with Sjögren’s) and not diagnosed with Sjogren’s (N = 22), with salivary hypofunction were included in the study. The processed blood specimen were blinded and sent to a lab. The unstimulated saliva was measured. Comparison of Anti-Ro/SSA and ANA were done.
Results: The mean age of Sjögren’s was 60.16 ± 12.6 and non- Sjögren’s was 60.71 ±13.2. The whole unstimulated saliva of Sjögren’s was 0.027 ± .03 ml/min and non- Sjögren’s were 0.022 ± 0.03ml/min. There were 86 % agreement with anti-Ro/SSA in Sjögren’s group compared to 87% in the non- Sjögren’s group to previous diagnosis of Sjögren’s (p=.000). The average years of diagnosis of Sjögren’s was 10 ± 2.3 years It is possible that SSA previously not identified in non- Sjögren’s group is detected in this study. The reported clinical significance by the blinded lab is hundred percent in both Sjögren’s and non-Sjögren’s group. The frequency and pattern of ANA antibodies are shown in the table below.
Conclusions: The Sjo system’s sensitivity in detection of anti-Ro/SSA and antinuclear antibodies is more with Speckled pattern (62%) at dilution concentration of 1:1280 (66.67%) in Sjögren’s as the frequency of occurrence of ANA Hep2 pattern is 90.5% in Sjögren’s. Sjo can be an instrumental tool for detection of the biomarkers and aid in diagnosis of Sjögren’s and other connective tissue diseases.
This study was sponsored by Bausch Health.