IADR Abstract Archives
Oral Microbiota Associated with Obstructive Sleep Apnea and Periodontitis
Objectives: To identify microorganisms of the oral microbiota of subjects with moderate and severe periodontitis and Obstructive Sleep Apnea (OSA), to determine if the oral microbiota is different in these subjects due to changes in the oral environment in the presence of both diseases.
Methods: Saliva, dental plaque and periodontal pocket samples were collected from four groups of subjects previously diagnosed (five subjects per group): healthy subjects, moderate and severe periodontitis subjects, OSA subjects and subjects with both diseases. These samples were cultured in Sabouraud agar and blood agar, incubated for 2 and 7 days at 37°C in aerobiosis and anaerobiosis chamber, respectively. Subsequently, each type of colony was identified and characterized macroscopically, microscopically and the global mass spectrometry tool called MALDI-TOF-MS was used on intact microbial cells, following the direct method of extracting proteins from colonies adding formic acid and then matrix solution.
Results: Healthy subjects samples had a high presence of gram-positive cocci such as S. mitis, S. parasanguinis, S. oralis, S. gordonii, and others as S. pneumoniae and Gemella morbillorum, A. naeslundii, A. georgiae and A. oris; P. histicola and P. micra and E. corrodens. Streptococcus spp., Veillonella spp, Fusobacterium spp., Propionibacterium spp. and Porphyromona spp. were identified in moderate and severe periodontitis subjects. Streptococcus spp., Actinomyces spp., Staphylococcus spp., Propionibacterium spp., Eubacterium brachy, and Candida albicans were identified from OSA subjects. By last, Propionibacterium spp., Capnocytophaga spp., Fusobacterium spp. Eubacterium brachy and Candida albicans were identified from subjects with both diseases.
Conclusions: These results broaden our knowledge of disease-specific differences in the oral microbiota associated with periodontitis and Obstructive Sleep Apnea (OSA) the presence of bacteria and yeasts as Propionibacterium spp., Capnocytophaga spp., Fusobacterium spp. Eubacterium brachy and Candida albicans in subjects with both diseases, it shows the importance to design new periodontal therapy according to the microbial community-associated.
Methods: Saliva, dental plaque and periodontal pocket samples were collected from four groups of subjects previously diagnosed (five subjects per group): healthy subjects, moderate and severe periodontitis subjects, OSA subjects and subjects with both diseases. These samples were cultured in Sabouraud agar and blood agar, incubated for 2 and 7 days at 37°C in aerobiosis and anaerobiosis chamber, respectively. Subsequently, each type of colony was identified and characterized macroscopically, microscopically and the global mass spectrometry tool called MALDI-TOF-MS was used on intact microbial cells, following the direct method of extracting proteins from colonies adding formic acid and then matrix solution.
Results: Healthy subjects samples had a high presence of gram-positive cocci such as S. mitis, S. parasanguinis, S. oralis, S. gordonii, and others as S. pneumoniae and Gemella morbillorum, A. naeslundii, A. georgiae and A. oris; P. histicola and P. micra and E. corrodens. Streptococcus spp., Veillonella spp, Fusobacterium spp., Propionibacterium spp. and Porphyromona spp. were identified in moderate and severe periodontitis subjects. Streptococcus spp., Actinomyces spp., Staphylococcus spp., Propionibacterium spp., Eubacterium brachy, and Candida albicans were identified from OSA subjects. By last, Propionibacterium spp., Capnocytophaga spp., Fusobacterium spp. Eubacterium brachy and Candida albicans were identified from subjects with both diseases.
Conclusions: These results broaden our knowledge of disease-specific differences in the oral microbiota associated with periodontitis and Obstructive Sleep Apnea (OSA) the presence of bacteria and yeasts as Propionibacterium spp., Capnocytophaga spp., Fusobacterium spp. Eubacterium brachy and Candida albicans in subjects with both diseases, it shows the importance to design new periodontal therapy according to the microbial community-associated.
