IADR Abstract Archives
Effectiveness of a Phytotherapeutic Irrigant Solution Against Enterococcus faecalis
Objectives: Enterococcus faecalis is the most frequently detected species in root canal treated teeth, and able to survive under starvation conditions. The purpose of this study was to evaluate in vitro the antibacterial activity of a tea tree oil based irrigating solution on Enterococcus faecalis, in platonic form, compared to sodium hypochlorite and chlorhexidine.
Methods: Enterococcus faecalis (ATCC 29212) was inoculated in Brain Heart Infusion Medium (BHI) at sterile 96-well cell culture plates with tea tree oil-based solution (TTO), 2% chlorhexidine gluconate (CLX) or 2.5% hypochlorite (NaOCl); unexposed Enterococcus faecalis were the control group (CT). All root canal irrigants were prepared at different dilutions of 10%, 25%, 50%, and 90%. The bacterial growth was evaluated using Epoch 2 Microplate Spectrophotometer(600nm) at 37°C, with measurements taken every 30 minutes for 6 hours to establish the growth-curve. The experiments were repeated 3 times independently. Statistics:ANOVA, Bonferroni post-test (n=9/group, p<0.05).
Results: The results showed similar anti-bacterial effect for TTO and NaOCl at 1h and 6h of incubation. CLX group was statistically less effective than the other groups at the first hour of incubation.
Conclusions: The Tea Tree Oil-based root canal irrigant showed an interesting effect over Enterococcus faecalis in this study. On the other hand, 2% chlorhexidine gluconate seemed to be just bacteriostatic when diluted, and its effectiveness should be further investigated.
Methods: Enterococcus faecalis (ATCC 29212) was inoculated in Brain Heart Infusion Medium (BHI) at sterile 96-well cell culture plates with tea tree oil-based solution (TTO), 2% chlorhexidine gluconate (CLX) or 2.5% hypochlorite (NaOCl); unexposed Enterococcus faecalis were the control group (CT). All root canal irrigants were prepared at different dilutions of 10%, 25%, 50%, and 90%. The bacterial growth was evaluated using Epoch 2 Microplate Spectrophotometer(600nm) at 37°C, with measurements taken every 30 minutes for 6 hours to establish the growth-curve. The experiments were repeated 3 times independently. Statistics:ANOVA, Bonferroni post-test (n=9/group, p<0.05).
Results: The results showed similar anti-bacterial effect for TTO and NaOCl at 1h and 6h of incubation. CLX group was statistically less effective than the other groups at the first hour of incubation.
Conclusions: The Tea Tree Oil-based root canal irrigant showed an interesting effect over Enterococcus faecalis in this study. On the other hand, 2% chlorhexidine gluconate seemed to be just bacteriostatic when diluted, and its effectiveness should be further investigated.
