IADR Abstract Archives
Healing of Intrabony Defects Following Reconstructive Surgery with Hyaluronic Acid
Objectives: Hyaluronic acid (HA) has been shown to enhance the proliferative and migratory properties of cell types involved in periodontal wound healing/regeneration. Additionally, HA may also stabilize the blood clot and reduce the inflammatory response, thus enhancing neovascularization and angiogenesis. However, at present it is unknown to what extent the use of HA during periodontal surgery may enhance periodontal wound healing/regeneration in intrabony defects.
To clinically and histologically evaluate the potential effects of HA alone or combined with a collagen matrix on the healing of intrabony defects.
Methods: Two-wall intrabony defects (5 mm wide, 5 mm deep) were surgically created at the distal and mesial aspects of mandibular premolars in four beagle dogs. The 16 defects were randomly treated as follows: a) open flap debridement (OFD) + HA (Hyadent, Regedent AG, Zürich, Switzerland) b) OFD + porcine collagen matrix (CM) (Fibrogide, Geistlich, Wolhusen, Switzerland), OFD + HA + CM (HA/CM) and OFD alone (control). At 2 months, the animals were euthanized and the tissues processed for histologic evaluation.
Results: The healing was uneventful at all sites. No adverse reactions such as suppuration, abscess formation or increased tooth mobility, were observed throughout the entire study period. The use of HA, CM and HA + CM yielded statistically significantly (p< 0.05) higher formation of cementum with inserting collagen fibers (i.e. 3.62±0.92 mm in the HA group, 2.36±0.84 mm in the CM group and 4.03±0.78 mm in the HA/CM group, respectively) compared with OFD alone (0.31±0.20 mm). No statistically significant differences were found in terms of bone formation between the 4 groups.
Conclusions: In intrabony defects, the additional application of HA, CM or HA+CM to OFD positively influences periodontal wound healing/regeneration.
To clinically and histologically evaluate the potential effects of HA alone or combined with a collagen matrix on the healing of intrabony defects.
Methods: Two-wall intrabony defects (5 mm wide, 5 mm deep) were surgically created at the distal and mesial aspects of mandibular premolars in four beagle dogs. The 16 defects were randomly treated as follows: a) open flap debridement (OFD) + HA (Hyadent, Regedent AG, Zürich, Switzerland) b) OFD + porcine collagen matrix (CM) (Fibrogide, Geistlich, Wolhusen, Switzerland), OFD + HA + CM (HA/CM) and OFD alone (control). At 2 months, the animals were euthanized and the tissues processed for histologic evaluation.
Results: The healing was uneventful at all sites. No adverse reactions such as suppuration, abscess formation or increased tooth mobility, were observed throughout the entire study period. The use of HA, CM and HA + CM yielded statistically significantly (p< 0.05) higher formation of cementum with inserting collagen fibers (i.e. 3.62±0.92 mm in the HA group, 2.36±0.84 mm in the CM group and 4.03±0.78 mm in the HA/CM group, respectively) compared with OFD alone (0.31±0.20 mm). No statistically significant differences were found in terms of bone formation between the 4 groups.
Conclusions: In intrabony defects, the additional application of HA, CM or HA+CM to OFD positively influences periodontal wound healing/regeneration.