IADR Abstract Archives
Effect of Folic Acid and BMP2 on Mesenchymal Stem Cells
Objectives: Worldwide incidence of bone disorders has increased in recent years. Bone grafts are the most frequent treatment, despite of its complications, such as rejection, transmission of diseases, limited availability and morbidity. In addition, bone grafts are osteoconductive but not osteoinductive. Tissue engineering aims to develop biological substitutes that restore the function of altered tissues.
Bone morphogenetic proteins (BMPs), specifically BMP2/4/6/7 have an important role in osteoblastic differentiation. BMP2 is approved to be used in humans.
On the other hand, multiple epidemiological studies have shown that folic acid (FA) and multivitamin supplements help to prevent neural tube defects (NTDs) and suggest a preventive role in the formation of orofacial clefts.
Although BMP2 and FA are important factors for bone formation and regeneration, its effect on mesenchymal cells (MSCs) remains controversial.
Therefore, the aim of this study is to compare the effect of FA and BMP2 on proliferation and morphology of MSCs.
Methods: Isolated and characterized MSCs from two different patients were treated with different concentrations of FA and BMP2 to evaluate phenotypic changes, by inverted microscopy and proliferation rate, by resazurin colorimetric assay. The experiments were performed by duplicate and pooled data analyzed. Statistical analysis was performed using ANOVA, Kruskall-Wallis and post hoc Scheffé.
Results: BMP2 (50-100ng / ml) did not generate morphological changes in the DPSCs from 7 to 21 days of treatment, nor significant reduction in cell proliferation.
Cells treated with 1.6mM and 0.8mM of FA were observed more elongated and less confluent than no treated cells; a significantly decreased proliferation were observed between treated and no treated cells, but no differences between the concentrations of FA used.
Conclusions: BMP2 (50-100ng/ml) did not induce changes in phenotype and proliferation, FA (1.6-0.8mM) induce morphological changes and reduce the proliferation of MSCs. Its effect on differentiation into mineralizing phenotype in vitro has to be determined.
Bone morphogenetic proteins (BMPs), specifically BMP2/4/6/7 have an important role in osteoblastic differentiation. BMP2 is approved to be used in humans.
On the other hand, multiple epidemiological studies have shown that folic acid (FA) and multivitamin supplements help to prevent neural tube defects (NTDs) and suggest a preventive role in the formation of orofacial clefts.
Although BMP2 and FA are important factors for bone formation and regeneration, its effect on mesenchymal cells (MSCs) remains controversial.
Therefore, the aim of this study is to compare the effect of FA and BMP2 on proliferation and morphology of MSCs.
Methods: Isolated and characterized MSCs from two different patients were treated with different concentrations of FA and BMP2 to evaluate phenotypic changes, by inverted microscopy and proliferation rate, by resazurin colorimetric assay. The experiments were performed by duplicate and pooled data analyzed. Statistical analysis was performed using ANOVA, Kruskall-Wallis and post hoc Scheffé.
Results: BMP2 (50-100ng / ml) did not generate morphological changes in the DPSCs from 7 to 21 days of treatment, nor significant reduction in cell proliferation.
Cells treated with 1.6mM and 0.8mM of FA were observed more elongated and less confluent than no treated cells; a significantly decreased proliferation were observed between treated and no treated cells, but no differences between the concentrations of FA used.
Conclusions: BMP2 (50-100ng/ml) did not induce changes in phenotype and proliferation, FA (1.6-0.8mM) induce morphological changes and reduce the proliferation of MSCs. Its effect on differentiation into mineralizing phenotype in vitro has to be determined.
