Inhibitory Capacity Triphala and Chlorhexidine Against Porphyromonas Gingivalis Prevotella Intermedia

Objectives: To determine the capacity of Triphala and 0.12% Chlorhexidine to inhibit the formation of Porphyromonas gingivalis (PG) and Prevotella intermedia (PI) colony forming units (CFU).
Methods: Pure ATCC strains of PG (LOT 7104515) and PI (LOT 1187-08-2) were obtained. The broth cultures of PG and PI ATCC turbidity was adjusted to 0.5 McFarland standard. To reduce the concentration of microorganisms, seven dilutions were made by obtaining 180 microliters (μl) of ringer's lactate and 20 μl of the McFarland. These were identified with negative numbers from -2 to -7. The dilutions were inoculated onto BD Brucella Blood Agar with Hemin and Vitamin K1, and identified accordingly from -2 to -7 "before”. Next, 2 ml of Triphala and 2 ml of 0.12% chlorhexidine were placed in the McFarland of PG and PI, allowing to stand for 10 minutes. Then, the dilutions were made and labelled as “after”. Each were inoculated onto BD Brucella Blood Agar with Hemin and Vitamin K1, identified “after”, 3 replications were performed. The plaques were placed in anaerobic conditions at 37 C⁰ for 5 days.
Results: For PG after adding 0.12% chlorhexidine, the average colony-forming units (CFU) at dilution -2 was 48 CFU, -3: 20 CFU, -4: 0 CFU. After adding Triphala, the mean colony-forming units at dilution -2: 88 CFU, -3: 52 CFU, -4: 25 CFU and -5: 0 CFU. For PI After adding 0.12% chlorhexidine, the mean CFU at dilution -2: 63 CFU, -3: 31 CFU, -4: 3 CFU and -5: 0 CFU. After adding Triphala was from -2 to -7: 0 CFU
Conclusions: Triphala was able to inhibit CFU in 93.69% for PG and 100% for PI. The 0.12% chlorhexidine was able to inhibit the CFU by 97.61% for PG and 96.67% for the PI.