Human Osteoblastic Cells Response to Bioceramic Endodontic Cements

Objectives: Bioceramic materials have been proposed as repair cements for endodontic treatments, in special regenerative endodontics. The aim of this study was to evaluate the biocompatibility and biological properties of new bioceramic material: Bio-C Repair (Angelus, Londrina, PR, Brazil) compared to Biodentine (Septodont, Saint Maur des Fosses, France) in human osteoblastic cells (Saos-2).
Methods: Zinc-eugenol sealer (ZOE) and unexposed cultures were the control group (positive and negative, respectively). Metabolic activity, cell migration and bioactivity were assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), wound-healing and alkaline phosphatase (ALP) assays, respectively. Cells were cultured in direct contact with the elute of each material through transwell inserts and exposed for 24h. Statistical differences were assessed using ANOVA and Bonferroni (p<0.05).
Results: The cells exposed to the materials had greater viability compared to the control group, the cell migration of Saos-2 in the Biodentine group was significantly increased than the Bio-C Repair and negative control groups (p<0.01). The highest ALP activity was observed in Bio-C group 24h of exposure to the materials significantly increased than control groups (p<0.05), moreover similar activity was observed in Biodentine and Bio-C Repair.
Conclusions: Both bioceramic cements have excellent biocompatibility. Bio-C Repair group showed statistically significant higher ALP activity compared to control, and similiar to Biodentine group.