IADR Abstract Archives
Biocompatibility of Biodentine: An Experimental Study
Objectives: The aim of endodontic treatment is to regenerate the periapical sector. BiodentineTM, (Septodont, Saint-Maur-des-Fossés, France) has been used since 20109; several authors have suggested that periapical extrusion of the material could trigger an inflammatory process, which might complicate or even prevent tissue repair. The aim of this study was to perform an in vivo evaluation of hematopoietic bone marrow response to Biodentine at 7 and 30 days post-implantation.
Methods: Biodentine was analyzed by Scanning Electron Microscopy (SEM), and surface chemical composition was determined by Energy-Dispersive Spectroscopy (EDS). Twenty male Wistar rats, 100 g body weight, were anesthetized i.p. with 0.1 ml/100gb.w. of Ketamine 50mg (injectable solution, Holliday, Argentina) and Xylazine 2% (sterile solution of Xylazine 2%, Argentina), and subjected to implantation of a silicone tube (0.2 mm x 2mm) containing BiodentineTM, mixed according to the manufacturer’s instructions. The animals were euthanized at 7 and 30 days post-implantation using 1g/1ml of sodium thiopental (Thiopental-Scott Cassara-Germany). The tibiae were resected, fixed in formalin buffer, radiographed, demineralized in formic acid, and processed for embedding in paraffin. Longitudinal histological sections were obtained and stained with H-E.
Results: Evaluation by SEM showed that Biodentine exhibited a rough surface; higher magnification allowed observing the formation of crystalline structures. Chemical composition was: O 47.88%; SI 5.55%; Ca 40.92; Zr 1.69%. Histologic examination at 7 days showed the presence of a mild inflammatory response and of woven bone tissue at both ends of the tubes in contact with the biomaterial. At 30 days, no inflammatory response or multinucleated giant cells were found, and laminar bone tissue was observed in contact with the biomaterial.
Conclusions: In this experimental model, no BiodentineTM particles were found in the bone marrow; though a mild inflammatory response was observed, it did not affect the repair process.
Methods: Biodentine was analyzed by Scanning Electron Microscopy (SEM), and surface chemical composition was determined by Energy-Dispersive Spectroscopy (EDS). Twenty male Wistar rats, 100 g body weight, were anesthetized i.p. with 0.1 ml/100gb.w. of Ketamine 50mg (injectable solution, Holliday, Argentina) and Xylazine 2% (sterile solution of Xylazine 2%, Argentina), and subjected to implantation of a silicone tube (0.2 mm x 2mm) containing BiodentineTM, mixed according to the manufacturer’s instructions. The animals were euthanized at 7 and 30 days post-implantation using 1g/1ml of sodium thiopental (Thiopental-Scott Cassara-Germany). The tibiae were resected, fixed in formalin buffer, radiographed, demineralized in formic acid, and processed for embedding in paraffin. Longitudinal histological sections were obtained and stained with H-E.
Results: Evaluation by SEM showed that Biodentine exhibited a rough surface; higher magnification allowed observing the formation of crystalline structures. Chemical composition was: O 47.88%; SI 5.55%; Ca 40.92; Zr 1.69%. Histologic examination at 7 days showed the presence of a mild inflammatory response and of woven bone tissue at both ends of the tubes in contact with the biomaterial. At 30 days, no inflammatory response or multinucleated giant cells were found, and laminar bone tissue was observed in contact with the biomaterial.
Conclusions: In this experimental model, no BiodentineTM particles were found in the bone marrow; though a mild inflammatory response was observed, it did not affect the repair process.