IADR Abstract Archives

Evaluation of 3D-printed TiO2-PCL Scaffolds for Bone Tissue Engineering

Objectives: A major challenge in bone tissue engineering is developing a scaffold that possesses adequate mechanical properties while maintaining sufficient biocompatibility. The incorporation of titanium dioxide into 3D-printed polycaprolactone scaffolds aimed to address this.
Methods: Polycaprolactone (Mn 45 kDa, sigma) was dissolved in dichloromethane at a concentration of 5%w/v. Titanium dioxide (rutile) was added to the solution at a weight ratio 3 wt% to PCL and homogenized using mechanical stirring. The prepared suspension was casted on petri dishes allowing the solvent to evaporate. After casting the samples into petri dish, samples were exposed to a vacuum to remove residual solvent. The obtained films were grinded and used for printing. The samples were 3D-printed at 135C, using a pressure of 2-4 bars and 4-6 mm/s printhead speed. The scaffolds were evaluated for their mechanical properties, cell attachment and proliferation. Dental pulp stem cells were harvested from extracted 3rd molars for in vitro tests.
Results: FTIR analysis confirmed the integrity of TiO2-PCL 3d-printed scaffolds. Physical properties, such as color change, further confirmed the successful integration of TiO2. SEM imaging showed an increase in cell attachment on the TiO2 scaffolds in comparison to the PCL control group. Cell proliferation after Day 3 was significantly greater for the scaffolds with TiO2. After Day 5, both the bare PCL scaffolds and TiO2 failed to further proliferate.
Conclusions: Titanium dioxide was successfully integrated with polycaprolactone for use in the 3D-printing of scaffolds for bone tissue engineering. An increase in cell attachment and early cell proliferation insinuates that the TiO2 incorporated PCL scaffolds show improved potential for bone tissue engineering.

IADR/AADR/CADR General Session
2020 IADR/AADR/CADR General Session (Washington, D.C., USA)
Washington, D.C., USA
2020
0719
Dental Materials 2:Polymer-based Materials
  • Paape, Soren  ( Marquette University School of Dentistry , Milwaukee , Wisconsin , United States )
  • Tongas, Nikita  ( Marquette University School of Dentistry , Milwaukee , Wisconsin , United States )
  • Rasoulianboroujeni, Morteza  ( Marquette University School of Dentistry , Milwaukee , Wisconsin , United States )
  • Yadegari, Amir  ( Marquette University School of Dentistry , Milwaukee , Wisconsin , United States )
  • Tayebi, Lobat  ( Marquette University School of Dentistry , Milwaukee , Wisconsin , United States )
  • NONE
    Poster Session
    Polymer-based Materials: 3D Printing