IADR Abstract Archives
Physicochemical and Biological Properties Assessment of Composites-enriched With Red Propolis
Objectives: Propolis is a natural compound produced by bees widely used, that has a plenty of biological and pharmacological properties and plays a promising role in dentistry. This study assessed the effect of Brazilian red propolis (BRP) on degree of conversion, hardness, roughness, flexural strength, sorption, solubility, antimicrobial activity, cell proliferation and cytotoxicity of one dental composite.
Methods: Ethyl acetate extract of BRP (EABRP) were analyzed by ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) to identify its biomakers. Three formulations of a bulk fill-flowable composite enriched with BRP (0.10, 0.15, 0.25%) were prepared and Filtek Bulk Fill Flow (3M/ESPE) was used as control group. Conversion degree (DC) was measured using near infrared spectroscopy, immediately and 24h after polymerization. Composite samples were assessed for Knoop hardness (KHN), roughness (Ra), flexural strength (FS), sorption (SO), solubility (SB) after 24h immersed in water, 37 oC. The antimicrobial activity was tested against Streptoccoccus mutans. Cytotoxicity and cell proliferation assays were carried out on fibroblasts cell line 3T3. Data were analyzed using one-way ANOVA and Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (α=0.05).
Results: The UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin and isoliquiritigenin and the isoflavonoids daidzein, formononetin and biochanin A. EABPR showed antioxidant activity (IC50 1 µg/mL). For all RP groups the means of KHN was 20.1; Ra = 21 nm; FS= 106,7 MPa; SO<25 µg/mL; SB<5 µg/mL with significant diference compared to control group (p<0.05). All experimental composite showed similar performance (p>0.05). BRP increased the DC (p<0.05) of experimental resins (72.1%-73.0%) compared to commercial composite (56,85%). After one hour 0.25% composite enriched with BRP inhibited bacterial growth by 8.8% (log CFU/mL). Additionally, all RP induced cell proliferation with no cytotoxicity on fibroblasts.
Conclusions: The composites enriched with BRP showed adequated physicochemical properties, antimicrobial activity and potential to assist on healing with no cytotoxicity. Thus this new composites have potential to be applied on restorative dentistry.
Methods: Ethyl acetate extract of BRP (EABRP) were analyzed by ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) to identify its biomakers. Three formulations of a bulk fill-flowable composite enriched with BRP (0.10, 0.15, 0.25%) were prepared and Filtek Bulk Fill Flow (3M/ESPE) was used as control group. Conversion degree (DC) was measured using near infrared spectroscopy, immediately and 24h after polymerization. Composite samples were assessed for Knoop hardness (KHN), roughness (Ra), flexural strength (FS), sorption (SO), solubility (SB) after 24h immersed in water, 37 oC. The antimicrobial activity was tested against Streptoccoccus mutans. Cytotoxicity and cell proliferation assays were carried out on fibroblasts cell line 3T3. Data were analyzed using one-way ANOVA and Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (α=0.05).
Results: The UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin and isoliquiritigenin and the isoflavonoids daidzein, formononetin and biochanin A. EABPR showed antioxidant activity (IC50 1 µg/mL). For all RP groups the means of KHN was 20.1; Ra = 21 nm; FS= 106,7 MPa; SO<25 µg/mL; SB<5 µg/mL with significant diference compared to control group (p<0.05). All experimental composite showed similar performance (p>0.05). BRP increased the DC (p<0.05) of experimental resins (72.1%-73.0%) compared to commercial composite (56,85%). After one hour 0.25% composite enriched with BRP inhibited bacterial growth by 8.8% (log CFU/mL). Additionally, all RP induced cell proliferation with no cytotoxicity on fibroblasts.
Conclusions: The composites enriched with BRP showed adequated physicochemical properties, antimicrobial activity and potential to assist on healing with no cytotoxicity. Thus this new composites have potential to be applied on restorative dentistry.
