IADR Abstract Archives
Short and Long-wavelengths Effects on Cell Metabolism During Restorative Procedures
Objectives: To evaluate the effect of short and long wavelengths on odontoblast-like cells during direct restorative procedures.
Methods: MDPC-23 odontoblast-like cells were seeded (104 cells/cm2), incubated for 12 h in complete medium. Then, the culture medium was replaced by DMEM supplemented with 0.5% bovine serum to induce metabolic stress. After 12 h incubation, a simulated restorative procedure was performed on the bottom of the 96-well plates. Therefore, the cells were irradiated first for 10 sec by the following wavelengths, blue with peak at 460 nm or red with peak at 620 nm (Valo-Experimental, Ultradent) with a 1000 mW/cm2 radiant emittance through an adhesive layer. Then, the cells were irradiated for more 20 sec but through a 2 mm thick composite build-up. Non-irradiated cells served as control. The cell metabolism was evaluated using MTT assay. The data (n=12 per group) were submitted to Kruskal-Wallis and Mann-Whitney tests (p=0.05).
Results: The simulated restorative procedures resulted in significant reduction of the cell mentalism when the blue LED was used (approximately 40% in average) (p<0.0001). No difference was observed between the control group and red LED group (p>0.05).
Conclusions: IIn conclusion, the restorative procedures using long wavelengths such as the red light did not cause any alteration of the metabolism of the cells, while the short wavelengths such as the blue light resulted in a significant reduction.
Methods: MDPC-23 odontoblast-like cells were seeded (104 cells/cm2), incubated for 12 h in complete medium. Then, the culture medium was replaced by DMEM supplemented with 0.5% bovine serum to induce metabolic stress. After 12 h incubation, a simulated restorative procedure was performed on the bottom of the 96-well plates. Therefore, the cells were irradiated first for 10 sec by the following wavelengths, blue with peak at 460 nm or red with peak at 620 nm (Valo-Experimental, Ultradent) with a 1000 mW/cm2 radiant emittance through an adhesive layer. Then, the cells were irradiated for more 20 sec but through a 2 mm thick composite build-up. Non-irradiated cells served as control. The cell metabolism was evaluated using MTT assay. The data (n=12 per group) were submitted to Kruskal-Wallis and Mann-Whitney tests (p=0.05).
Results: The simulated restorative procedures resulted in significant reduction of the cell mentalism when the blue LED was used (approximately 40% in average) (p<0.0001). No difference was observed between the control group and red LED group (p>0.05).
Conclusions: IIn conclusion, the restorative procedures using long wavelengths such as the red light did not cause any alteration of the metabolism of the cells, while the short wavelengths such as the blue light resulted in a significant reduction.
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