IADR Abstract Archives
Development of Ex-vivo Biofilm Model for Toothpaste Efficacy Assessment
Objectives:
The objective was to develop an ex-vivo model to assess efficacy of a zinc containing toothpaste in reducing oral bacterial biofilm formation using samples collected from multiple locations round the mouth versus a control toothpaste without zinc.
Methods: Bacterial samples representing the whole mouth (Teeth, Gum, Tongue and Cheek) were collected from subjects in good general and oral health using a swab. The site-specific samples were homogenised, washed and suspended in phosphate buffered saline (PBS) and optical density (OD) adjusted to 1.
Toothpaste products:
Test: Fluoride containing toothpaste with 2% zinc citrate trihydrate
Control: Fluoride containing toothpaste without zinc citrate trihydrate
Pellicle coated hydroxyapatite discs were treated with toothpastes or water in 12 well plates for 1 minute and rinsed with PBS. The treated and rinsed discs were transferred into wells containing fresh media, inoculated with the prepared bacterial whole mouth sample suspension and incubated for 20 hours at 37 degrees celsius under anaerobic conditions for biofilm formation. The total viable count (TVC) for aerobic and anaerobic bacteria was determined by spiral plating onto agar and incubated for 72 hours.
Statistical analysis performed by comparing test toothpaste with control toothpaste using the Tukey-Kramer test implemented in SAS. Data carrying the same letter are not significantly different (p<0.05)
Results:
The zinc containing toothpaste was significantly better in reducing oral bacterial biofilm formation than the control toothpaste (p<0.05).
Conclusions:
We have developed a robust and rapid ex-vivo biofilm method using bacteria collected from multiple locations round the mouth to differentiate the effect of toothpaste formulations on reducing oral bacteria biofilm formation.
The objective was to develop an ex-vivo model to assess efficacy of a zinc containing toothpaste in reducing oral bacterial biofilm formation using samples collected from multiple locations round the mouth versus a control toothpaste without zinc.
Methods: Bacterial samples representing the whole mouth (Teeth, Gum, Tongue and Cheek) were collected from subjects in good general and oral health using a swab. The site-specific samples were homogenised, washed and suspended in phosphate buffered saline (PBS) and optical density (OD) adjusted to 1.
Toothpaste products:
Test: Fluoride containing toothpaste with 2% zinc citrate trihydrate
Control: Fluoride containing toothpaste without zinc citrate trihydrate
Pellicle coated hydroxyapatite discs were treated with toothpastes or water in 12 well plates for 1 minute and rinsed with PBS. The treated and rinsed discs were transferred into wells containing fresh media, inoculated with the prepared bacterial whole mouth sample suspension and incubated for 20 hours at 37 degrees celsius under anaerobic conditions for biofilm formation. The total viable count (TVC) for aerobic and anaerobic bacteria was determined by spiral plating onto agar and incubated for 72 hours.
Statistical analysis performed by comparing test toothpaste with control toothpaste using the Tukey-Kramer test implemented in SAS. Data carrying the same letter are not significantly different (p<0.05)
Results:
The zinc containing toothpaste was significantly better in reducing oral bacterial biofilm formation than the control toothpaste (p<0.05).
Conclusions:
We have developed a robust and rapid ex-vivo biofilm method using bacteria collected from multiple locations round the mouth to differentiate the effect of toothpaste formulations on reducing oral bacteria biofilm formation.