IADR Abstract Archives
Physical Properties and Anticariogenic Effec of Ursolic-acid Containing Resin Cements
Objectives: This study is designed to evaluate the physical properties and anti-bacterial activity of resin cement containing ursolic acid (UA) and determine the optimal concentration of UA.
Methods: Five types of experimental resin cement were prepared according to UA concentration (0, 0.1, 0.5, 1.0 and 2.0 wt%). Flexural strength and film thickness was measured to confirm whether the resin was appropriate under ISO criteria as a dental luting material based on ISO 4049. Fifty extracted human molars were prepared with a 2 mm x 4 mm x 2 mm (height x width x depth) cavity. Tescera inlays were cemented with experimental resins for each sample. Acid-resistant nail varnish was applied, except for the area 2 mm around the restoration. Artificial caries was induced for six days through Streptococcus mutans (ATCC25175). To evaluate the caries progression, Quantitative Light-induced Fluorescence (QLF) was used to compare the before and after caries induction. In vitro cytotoxicity was also evaluated based on ISO 10993-5 with L929 mouse fibroblast (Korean Cell line Bank, Korea). One-way ANOVA followed by the Dunnett correction were used to statistically analyze the data.
Results: In all groups, the mechanical property of flexural strength, film thickness and in vitro cytotoxicity were satisfied for ISO criteria (p>0.05). On ΔF (-%) and ΔQ (-%●Px) values as QLF parameters, there was a tendency of being lower in groups of resin cement containing higher concentration of UA. Resin cement containing UA of more than 0.5% significantly inhibited caries in the area around restoration (p<0.05). There was no difference between the groups containing UA of more than 0.5%.
Conclusions: Within the limitations of this study, resin cement containing UA up to 2% satisfied the ISO criteria for flexural strength, film thickness and in vitro cytotoxicity, and resin cement containing UA of more than 0.5% showed an anti-caries effect.
Methods: Five types of experimental resin cement were prepared according to UA concentration (0, 0.1, 0.5, 1.0 and 2.0 wt%). Flexural strength and film thickness was measured to confirm whether the resin was appropriate under ISO criteria as a dental luting material based on ISO 4049. Fifty extracted human molars were prepared with a 2 mm x 4 mm x 2 mm (height x width x depth) cavity. Tescera inlays were cemented with experimental resins for each sample. Acid-resistant nail varnish was applied, except for the area 2 mm around the restoration. Artificial caries was induced for six days through Streptococcus mutans (ATCC25175). To evaluate the caries progression, Quantitative Light-induced Fluorescence (QLF) was used to compare the before and after caries induction. In vitro cytotoxicity was also evaluated based on ISO 10993-5 with L929 mouse fibroblast (Korean Cell line Bank, Korea). One-way ANOVA followed by the Dunnett correction were used to statistically analyze the data.
Results: In all groups, the mechanical property of flexural strength, film thickness and in vitro cytotoxicity were satisfied for ISO criteria (p>0.05). On ΔF (-%) and ΔQ (-%●Px) values as QLF parameters, there was a tendency of being lower in groups of resin cement containing higher concentration of UA. Resin cement containing UA of more than 0.5% significantly inhibited caries in the area around restoration (p<0.05). There was no difference between the groups containing UA of more than 0.5%.
Conclusions: Within the limitations of this study, resin cement containing UA up to 2% satisfied the ISO criteria for flexural strength, film thickness and in vitro cytotoxicity, and resin cement containing UA of more than 0.5% showed an anti-caries effect.
