IADR Abstract Archives
An Enzyme Toothpaste for the Removal of Tough Stains
Objectives:
To evaluate the invitro whitening efficacy of a toothpaste containing a perhydrolase enzyme and 0.1% hydrogen peroxide (HP) for the instu generation of peracetic acid (PAA) during brushing. Methods to generate tough stains on bovine teeth with commonly consumed beverages and tobacco were developed. The enzyme toothpaste was evaluated for its ability to remove these invitro generated stains compared to a negative control toothpaste. In addition the stain removal efficacy of this toothpaste was measured against commercial whitening toothpastes .
Methods:
Bovine teeth mounted on an acrylic resin were etched. Staining of bovine teeth was performed for two weeks using a bacterial broth containing common beverages or tobacco. Bovine teeth stained with coffee and tea were also commercially sourced. Teeth were brushed until initial L* values approached those of naturally stained human teeth (60 to 68). Twelve teeth were mounted (4 per tray) and baseline L*, a*, and b* values were measured with a spectrophotometer. Teeth were brushed for two minutes with a toothpaste – artificial saliva slurry 1:1 (w/w). This process was repeated a total of 14 times to model one week of twice daily brushing. After 14 brushing cycles, the L*, a*, and b* values were measured again. The Whiteness index, ΔW* (where W*= ((L*-100)2 + a*2 + b*2)1/2) was reported after 14 brushing treatments. Analysis of variance using baseline as a covariate was performed to compare the mean ΔW* values for each product with p<0.05 indicating statistically significant differences between products.
Results:
The test toothpaste containing the perhydrolase enzyme and 0.1% HP whitening system delivers statistically significantly (p<0.05) better in-vitro whitening efficacy compared to the negative control toothpaste. In addition, the enzyme toothpaste gave statistically superior efficacy in brushing studies versus other commercial whitening toothpastes.
Conclusions:
The model developed can differentiate toothpaste’s ability to reduce stains. The enzyme toothpaste effectively reduced stained caused by tobacco and common beverages. The enzyme toothpaste showed superior stain removal efficacy versus commercial whitening toothpastes.
To evaluate the invitro whitening efficacy of a toothpaste containing a perhydrolase enzyme and 0.1% hydrogen peroxide (HP) for the instu generation of peracetic acid (PAA) during brushing. Methods to generate tough stains on bovine teeth with commonly consumed beverages and tobacco were developed. The enzyme toothpaste was evaluated for its ability to remove these invitro generated stains compared to a negative control toothpaste. In addition the stain removal efficacy of this toothpaste was measured against commercial whitening toothpastes .
Methods:
Bovine teeth mounted on an acrylic resin were etched. Staining of bovine teeth was performed for two weeks using a bacterial broth containing common beverages or tobacco. Bovine teeth stained with coffee and tea were also commercially sourced. Teeth were brushed until initial L* values approached those of naturally stained human teeth (60 to 68). Twelve teeth were mounted (4 per tray) and baseline L*, a*, and b* values were measured with a spectrophotometer. Teeth were brushed for two minutes with a toothpaste – artificial saliva slurry 1:1 (w/w). This process was repeated a total of 14 times to model one week of twice daily brushing. After 14 brushing cycles, the L*, a*, and b* values were measured again. The Whiteness index, ΔW* (where W*= ((L*-100)2 + a*2 + b*2)1/2) was reported after 14 brushing treatments. Analysis of variance using baseline as a covariate was performed to compare the mean ΔW* values for each product with p<0.05 indicating statistically significant differences between products.
Results:
The test toothpaste containing the perhydrolase enzyme and 0.1% HP whitening system delivers statistically significantly (p<0.05) better in-vitro whitening efficacy compared to the negative control toothpaste. In addition, the enzyme toothpaste gave statistically superior efficacy in brushing studies versus other commercial whitening toothpastes.
Conclusions:
The model developed can differentiate toothpaste’s ability to reduce stains. The enzyme toothpaste effectively reduced stained caused by tobacco and common beverages. The enzyme toothpaste showed superior stain removal efficacy versus commercial whitening toothpastes.