IADR Abstract Archives
Silicate-based Dentifrices Against Enamel Demineralization: In vitro Study
Objectives: The aim of this investigation was to evaluate the potential of dentifrices containing silicate-based technologies for remineralizing artificial caries lesions of bovine enamel.
Methods: Specimens (n=60) were prepared from extracted bovine teeth and randomly distributed into four groups of 15 specimens each according to surface microhardness: G1(Dentifrice without fluoride, Colgate); G2 (Silicate-based experimental dentifrice with fluoride, Dental Clean); G3(dentifrice with fluoride, Regenerate Enamel Science™) and G4(Silicate-based dentifrice with fluoride, Sensodyne Repair & Protect). The surface of the specimens was divided in into three parts of same size to observe sound, demineralized and treated (potential remineralized) enamel areas. One-third of the specimens surfaces were coated twice with nail varnish to create control areas (sound and demineralized). The specimens were submitted to a pH cycling model for 7 days. Briefly, the enamel samples were exposed to dentifrice slurry (dentifrice:deonized water, 1:3 – w/w; 2mL/enamel specimen) for 1 minute, twice a day, before and after immersion into to demineralization solution (for 6 h), and subsequently the specimens were immersed into a remineralization solution for 18 h. The magnitude of remineralization was measured by surface microhardness (%SMHR) and Quantitative Light-induced Fluorescence (QLF, Qraycam Pro™, ΔFRE)
Results: All groups showed statistically significant differences regardless the method used for measuring remineralization (p<0.05). Mean±SD remineralization values (%SMHR) by surface microhardness for G1, G2, G3 and G4 were: 2.1 ± 1.5; 23.56 ± 8.2; 18.78 ± 4.27 and 14.14 ± 2.06, respectively. For QLF, the remineralization values (ΔFRE) for these groups were: -5.10 ± 3.40; 11.28 ± 5.02; 5.57 ± 6.05 and 2.15 ± 4.54, respectively. A statistically significant positive correlation was observed between these two ways of measuring remineralization (r=0,529, p<0,01).
Conclusions: It can be concluded that the silicate-based experimental dentifrice with fluoride (G2, Dental Clean) showed the best performance for remineralizing artificial caries in bovine enamel.
Methods: Specimens (n=60) were prepared from extracted bovine teeth and randomly distributed into four groups of 15 specimens each according to surface microhardness: G1(Dentifrice without fluoride, Colgate); G2 (Silicate-based experimental dentifrice with fluoride, Dental Clean); G3(dentifrice with fluoride, Regenerate Enamel Science™) and G4(Silicate-based dentifrice with fluoride, Sensodyne Repair & Protect). The surface of the specimens was divided in into three parts of same size to observe sound, demineralized and treated (potential remineralized) enamel areas. One-third of the specimens surfaces were coated twice with nail varnish to create control areas (sound and demineralized). The specimens were submitted to a pH cycling model for 7 days. Briefly, the enamel samples were exposed to dentifrice slurry (dentifrice:deonized water, 1:3 – w/w; 2mL/enamel specimen) for 1 minute, twice a day, before and after immersion into to demineralization solution (for 6 h), and subsequently the specimens were immersed into a remineralization solution for 18 h. The magnitude of remineralization was measured by surface microhardness (%SMHR) and Quantitative Light-induced Fluorescence (QLF, Qraycam Pro™, ΔFRE)
Results: All groups showed statistically significant differences regardless the method used for measuring remineralization (p<0.05). Mean±SD remineralization values (%SMHR) by surface microhardness for G1, G2, G3 and G4 were: 2.1 ± 1.5; 23.56 ± 8.2; 18.78 ± 4.27 and 14.14 ± 2.06, respectively. For QLF, the remineralization values (ΔFRE) for these groups were: -5.10 ± 3.40; 11.28 ± 5.02; 5.57 ± 6.05 and 2.15 ± 4.54, respectively. A statistically significant positive correlation was observed between these two ways of measuring remineralization (r=0,529, p<0,01).
Conclusions: It can be concluded that the silicate-based experimental dentifrice with fluoride (G2, Dental Clean) showed the best performance for remineralizing artificial caries in bovine enamel.