IADR Abstract Archives
Antifungal Activity of Croton Draconoides on Candida Albicans ATCC 10231
Objectives: To determine the antifungal activity of the Croton draconoides (Muell-Arg) against Candida albicans.
Methods: 200mg of Croton draconoides (pure dehydrated extract of grade blood - Lab. Angisa, Iquitos) and nystatin 100000 UI/ml (in solution, Lab Medifarma) that was used as a positive control group and they were compared with Candida albicans ATCC 10231. The antifungal activity of the Croton was determined by 02 methods: Agar Diffusion (Kirby-Bauer Method) and Well Diffusion method (drilling in agar gel, Alvarez et al. 2005) at 103 UFC/ml cell concentration in agar and incubated at 35°C. It was placed in wells at concentrations: 175, 250 and 500mg/ml of Croton and 500mg/ml at different times.
Results: At 24 hours, the highest degree of inhibition of Croton draconoides on nystatin was observed. The concentration of 500mg/ml of Croton showed 28.2mm diameter of inhibition halos, compared to nystatin with 18.7mm and the concentration of 175mg/ml of Croton showed 17mm diameter of inhibition halos, relatively equal to the inhibition diameter of nystatin with the aforementioned concentration. On Mann Whitney's test, it was determined that nystatin 500mg/ml had a greater antifungal effect on Croton draconoides 175mg/ml, unlike Croton draconoides 250 and 500mg/ml that showed greater activity on nystatin at any of its concentrations (p<0.05). The Kruskal-Wallis test shown that Croton draconoides observed different antifungal activity at concentrations of 175, 250 and 500mg/ml, being greater the inhibitory capacity of Candida growth when higher is the Croton concentration.
Conclusions: Croton draconoides had a higher antifungal activity compared to nystatin.
Methods: 200mg of Croton draconoides (pure dehydrated extract of grade blood - Lab. Angisa, Iquitos) and nystatin 100000 UI/ml (in solution, Lab Medifarma) that was used as a positive control group and they were compared with Candida albicans ATCC 10231. The antifungal activity of the Croton was determined by 02 methods: Agar Diffusion (Kirby-Bauer Method) and Well Diffusion method (drilling in agar gel, Alvarez et al. 2005) at 103 UFC/ml cell concentration in agar and incubated at 35°C. It was placed in wells at concentrations: 175, 250 and 500mg/ml of Croton and 500mg/ml at different times.
Results: At 24 hours, the highest degree of inhibition of Croton draconoides on nystatin was observed. The concentration of 500mg/ml of Croton showed 28.2mm diameter of inhibition halos, compared to nystatin with 18.7mm and the concentration of 175mg/ml of Croton showed 17mm diameter of inhibition halos, relatively equal to the inhibition diameter of nystatin with the aforementioned concentration. On Mann Whitney's test, it was determined that nystatin 500mg/ml had a greater antifungal effect on Croton draconoides 175mg/ml, unlike Croton draconoides 250 and 500mg/ml that showed greater activity on nystatin at any of its concentrations (p<0.05). The Kruskal-Wallis test shown that Croton draconoides observed different antifungal activity at concentrations of 175, 250 and 500mg/ml, being greater the inhibitory capacity of Candida growth when higher is the Croton concentration.
Conclusions: Croton draconoides had a higher antifungal activity compared to nystatin.
