IADR Abstract Archives
Titanium And Zirconia Particles Activate Inflammatory Response In Human Macrophages
Objectives: The materials released from dental implants and overlaying dental constructions contribute to the inflammatory reactions associated with peri-implantitis by stimulating inflammasome activation and secretion in macrophages. The aim of this study was to examine cytotoxic and proinflammatory effects, including inflammasome activation, of materials typically used in dental implants in an in vitro model. For this purpose, we characterized the effects of titanium and zirconia particles on the biological behavior of human macrophages on cell viability and expression of proinflammatory cytokines.
Methods: Human macrophages were exposed to different particles of titanium (Ti) and zirconia ceramic (ZrO2) at ≤ 5µm particle size in a cell-culture assay. Cytotoxicity was determined using MTT assays and cytokine expression of TNF-α, IL-1β and IL-6 was evaluated at the mRNA level by RT-PCR. Data were analyzed using student t-test and ANOVA (p < 0.05).
Results: Macrophage viability was severely increased when exposed to higher concentrations of titanium and zirconia particles (≥107) compared to the untreated controls (p<0.05). Cultured macrophages challenged with zirconia or titanium particles expressed increased mRNA for inflammatory cytokines TNF-α, IL-1β and IL-6 (»2.5-fold upregulated). However, zirconia particle-induced pro-inflammatory gene expression was lower than that induced by titanium particles (p<0.05).
Conclusions: In conclusion, our data suggest that zirconia ceramic particles are less bioactive than titanium particles.
Methods: Human macrophages were exposed to different particles of titanium (Ti) and zirconia ceramic (ZrO2) at ≤ 5µm particle size in a cell-culture assay. Cytotoxicity was determined using MTT assays and cytokine expression of TNF-α, IL-1β and IL-6 was evaluated at the mRNA level by RT-PCR. Data were analyzed using student t-test and ANOVA (p < 0.05).
Results: Macrophage viability was severely increased when exposed to higher concentrations of titanium and zirconia particles (≥107) compared to the untreated controls (p<0.05). Cultured macrophages challenged with zirconia or titanium particles expressed increased mRNA for inflammatory cytokines TNF-α, IL-1β and IL-6 (»2.5-fold upregulated). However, zirconia particle-induced pro-inflammatory gene expression was lower than that induced by titanium particles (p<0.05).
Conclusions: In conclusion, our data suggest that zirconia ceramic particles are less bioactive than titanium particles.
