IADR Abstract Archives
Effects of Colloidal Platinum Nanoparticles on Dental Pulp
Objectives: The therapeutic role of Colloidal Platinum Nanoparticles (CPN) in pulmonary inflammation has been a subject of interest. However, little is known about the effects of CPN when applied locally. The purpose of this study was to investigate the healing response of exposed dental pulp after direct pulp capping with CPN.
Methods: Direct pulp capping was performed in 36 maxillary first molars of 18 Wister rats. The pulps of the molars were intentionally exposed and were randomly subjected to the following groups (n=12): CPN application followed by 4-META/MMA-TBB resin (Resin cement Super Bond: SB) application – CPN+SB; Mineral trioxide aggregate (MTA) application followed by SB application – MTA+SB; and SB application. Pulpal responses were evaluated after 3, 7 and 28 days. Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney U tests. The significance level was set at .05.
Results: After day 3, inflammatory cell infiltration was significantly lower in MTA+SB and CPN+SB, compared to SB. Numerous blood vessels were observed in CPN+SB indicating rapid angiogenesis. After day 7, a thin layer of mineralized tissue was observed in all the groups. After day 28, complete closure of pulp exposure with mineralized tissue was observed in MTA+SB and CPN+SB, the latter produced the thickest mineralized tissue layer.
Conclusions: The results of this study demonstrated that CPN showed favourable healing response in rat’s dental pulp which was comparable to the current “gold standard” pulp capping material MTA. However, further studies are required to determine the mechanism of action, optimum dosage and suitable carrier of CPN.
Methods: Direct pulp capping was performed in 36 maxillary first molars of 18 Wister rats. The pulps of the molars were intentionally exposed and were randomly subjected to the following groups (n=12): CPN application followed by 4-META/MMA-TBB resin (Resin cement Super Bond: SB) application – CPN+SB; Mineral trioxide aggregate (MTA) application followed by SB application – MTA+SB; and SB application. Pulpal responses were evaluated after 3, 7 and 28 days. Statistical analysis was performed using the Kruskal-Wallis and Mann-Whitney U tests. The significance level was set at .05.
Results: After day 3, inflammatory cell infiltration was significantly lower in MTA+SB and CPN+SB, compared to SB. Numerous blood vessels were observed in CPN+SB indicating rapid angiogenesis. After day 7, a thin layer of mineralized tissue was observed in all the groups. After day 28, complete closure of pulp exposure with mineralized tissue was observed in MTA+SB and CPN+SB, the latter produced the thickest mineralized tissue layer.
Conclusions: The results of this study demonstrated that CPN showed favourable healing response in rat’s dental pulp which was comparable to the current “gold standard” pulp capping material MTA. However, further studies are required to determine the mechanism of action, optimum dosage and suitable carrier of CPN.