Antifungal Effect of Caesalpinia spinosa on Candida spp.

Objectives: The aim of this study was to evaluate the antifungal effect of Caesalpinia spinosa butanolic, chloroformic and aqueous partitions on Candida albicans, Candida krusei, Candida tropicalis and Candida Parapsilopsis.
Methods: Caesalpinia spinosa was completely solubilized with methanol (MeOH) and chloroform (CHCl3); water (H2O) was added, followed by the solubilized crude extract. Portions of CHCl3 were added to the column containing the sample, all the concentrated chloroformic phases were called chloroform residue. In another column butanol (BuOH) was added with the sample, the butanolic phases was concentrated until obtaining a solid which was called BuOH residue. The aqueous phase was removed and frozen and lyophilization, which was called aqueous residue. Partitions (chloroformic, butanolic and aqueous) were diluted in dimethylsulfoxide DMSO (20 and 40 mg/ml). Nystatin and Clorhexidine as positive controls.The antifungal effect was evaluated by disc diffusion method on C albicans, C. tropicalis, C. krusei and C. parapsilopsis. This study was done in three different samples twice.
Results: Chloroformic residue 20mg/mL had no effect on Candida spp. Halos 0 mm, 40mg/mLC.albicans :11.17 ± 1.10 mm, C. tropicalis: 12.06 ± 1.33 mm, C. krusei: 0 mm, C. parapsilopsis: 11.3 ± 0.39 mm.Butanolic residue had positive halos of inhibition on C albicans: 20mg/ml: 18.43±2.40mm, 40mg/ml: 20.88±1.39mm; C. tropicalis: 20mg/ml: 18.60±1.68mm,40mg/ml: 20.96±1.09 mm; C krusei: 20mg/ml: 17.62± 2.03 mm, 40mg/ml: 20.32±0.94 mm; C parapsilopsis: 20mg/ml: 17.57±1.17mm, 40mg/ml: 20.15±1.23mm. Aqueous residue had positive halos of inhibition on C albicans: 20mg/ml: 16.07±1.16mm, 40mg/ml: 18.48±1.16mm; C tropicalis: 20mg/ml: 17.36±2.58mm, 40mg/ml: 21.12 ± 2.04 mm; C krusei: 20mg/ml: 15.32±1.09mm,40mg/ml: 19.47±1.61mm; C parapsilopsis: 20mg/ml: 15.97±1.21mm,40mg/ml: 19.88±1.87mm. Nystatin on C albicans: 13.53±0.70mm, C tropicalis: 12.08±0.63mm, C krusei: 12.90 ±0.78mm, C parapsilopsis: 13.55±0.82mmClorhexidine 0.125%: C albicans: 18.95±3.4mm, C tropicalis: 22.04±2.82mm, C krusei: 27.42±1.80mm, C parapsilopsis: 22.52±1.57mm.
Conclusions: Butanolic and aqueous partitions of Caesalpinia spinosa have antifungal effect on C albicans, C. tropicalis, C. krusei and C. parapsilopsis