IADR Abstract Archives
Mechanical, Thermal and Morphological Characterization of 3D-Printed Magnetic Hydrogel
Objectives: The aim of the current study is the characterization of the 3D-printed scaffold of superparamagnetic iron oxide nanoparticles (SPION)-hydrogel for soft tissue engineering and guided tissue regeneration (GTR).
Methods: The bioink was prepared by adding porcine gelatin (8 wt.%), elastin (2 wt.%) and sodium hyaluronate (0.5 wt.%) in deionized water at 50. Next, the sonicated carboxylic SPIONs were added to the above solution and mixed overnight at 40. The bioink was printed at a material container temperature of 30-32, and then the 3D-printed membranes were cross-linked by soaking them in 5mg/ml EDC and 0.5 mg/ml NHS in 70% ethanol for 3 hr. The mechanical properties of the scaffolds were evaluated using a universal testing machine (Shimadzu, Japan). Moreover, the surface morphology and roughness were analyzed using the 3D Laser Measuring Microscope. The glass transition (Tg) was also measured via differential scanning calorimetry (DSC) curves.
Results: The result showed that the mechanical properties of scaffolds were improved after the addition of SPIONs. The glass temperature of the SPION scaffold increased slightly with the addition of iron oxide, indicating the improvement of thermal stability. Furthermore, the laser microscope showed that the roughness of scaffolds with and without SPIONs was different. The cell viability assay using dental pulp stem cells indicated that the scaffolds have desirable in vitro biocompatibility.
Conclusions: The proposed 3D printed scaffold can be used in future work for magnetic resonance visualization of the scaffolds after in vivo implantation.
Methods: The bioink was prepared by adding porcine gelatin (8 wt.%), elastin (2 wt.%) and sodium hyaluronate (0.5 wt.%) in deionized water at 50. Next, the sonicated carboxylic SPIONs were added to the above solution and mixed overnight at 40. The bioink was printed at a material container temperature of 30-32, and then the 3D-printed membranes were cross-linked by soaking them in 5mg/ml EDC and 0.5 mg/ml NHS in 70% ethanol for 3 hr. The mechanical properties of the scaffolds were evaluated using a universal testing machine (Shimadzu, Japan). Moreover, the surface morphology and roughness were analyzed using the 3D Laser Measuring Microscope. The glass transition (Tg) was also measured via differential scanning calorimetry (DSC) curves.
Results: The result showed that the mechanical properties of scaffolds were improved after the addition of SPIONs. The glass temperature of the SPION scaffold increased slightly with the addition of iron oxide, indicating the improvement of thermal stability. Furthermore, the laser microscope showed that the roughness of scaffolds with and without SPIONs was different. The cell viability assay using dental pulp stem cells indicated that the scaffolds have desirable in vitro biocompatibility.
Conclusions: The proposed 3D printed scaffold can be used in future work for magnetic resonance visualization of the scaffolds after in vivo implantation.
