Formulate and Characterization of New Experimental Antibacterial Dental Bonding Agents

Objectives: To formulate and characterize new experimental antibacterial dental bonding agents.
Methods: The synthesized self-etching methacrylamide monomer MAUAEP containing phosphonic acid and the antibacterial dimethacrylate monomer C2DC12DMA containing DABCO dications have been previously reported. The adhesive dimethacrylate monomer 4,4-Bis[(4-(2-methacryloyloxy)ethoxy)phenyl]-pentanoic acid (BMEPPA) has been synthesized. A two-bottle bonding agent (Control) was formulated as follows: Bottle-A (primer): 7.5% MAUAEP, 7.5% DEGDA, 24%HEMA, 30% EtOH, 30% H₂O, 1% Photo Initiator; and Bottle-B (adhesive): 15%BisGMA, 30%TEGDMA, 20%UEDMA, 24%HEMA and 10%BMEPPA, 1% photo initiator. Three experimental antibacterial adhesives were formulated by adding 5%, 7.5%, and 10% of C2DC12DMA to the control adhesive. Experimental primer is the same as control primer. Antibacterial activity of light-cured adhesive discs (6 mm diameter, 2 mm thick, n=5) against S. mutans biofilm were tested using modified direct-contact biofilm assay and drop plating. Microtensile bonding strength (MTBS) of experimental bonding agents was tested on the dentin of extracted human teeth (n=12) after 24h storage in de-ionized water. The data were analyzed using ANOVA and Tukey test (α=0.05).
Results: Results: See Table 1.
The adhesives of experimental antibacterial dental bonding agents have significantly higher inhibitory effects against S. mutans biofilm than the control adhesive (P<0.05), and their biofilm-inhibitory effect increases with increase of the content of antibacterial monomer. The experimental antibacterial dental bonding agents have similar MTBS to the control (P>0.05).
Conclusions: The new experimental antibacterial dental bonding agents containing 7.5% or higher amount of antibacterial dimethacrylate monomer C2DC12DMA has significant antibacterial effect and good bonding strength.