IADR Abstract Archives
Role of Glass Ionomer with CPP-ACP in Secondary Caries Formation: In Vitro Study
Objectives: This in vitro study evaluated effects of a glass ionomer with calcium phosphopeptide-amorphous calcium phosphate (GI-CPP-ACP) compared with a glass ionomer without CPP-ACP (GI) on secondary caries formation.
Methods: 10 caries-free human molars underwent prophylaxis and cavity preparation in buccal and lingual surfaces. GI (GC Fuji II, GC Corp) and GI-CPP-ACP (GC Fuji VII, GC Corp) were placed in coronal buccal and lingual cavity preparations, per manufacturer's instructions. Acid-resistant varnish was applied, leaving 1mm windows of exposed surface enamel around restored cavities. Specimens were thermocycled in synthetic saliva for 24hrs. Artificial enamel caries were created (modified ten Cate method for 10d: alternating 8hrs demineralization followed by 16hrs remineralization). Longitudinal sections (3 sections/specimen, 60 lesions/group) were obtained. Cavity wall lesions presence and adjacent enamel surface lesion depths were determined using polarized light microscopy (water imbibition, ANOVA, DMR).
Results: Mean depths for adjacent enamel surface lesions were: GI Group: 352 ± 36um, and GI-CPP-ACP Group: 217 ± 23 um (P<.05). Enamel surface lesion depth reduction with GI-CPP-ACP restorations compared with GI restorations was 38% (P<.05). Wall lesions were confined to the superficial aspect of cavity walls, and present at 13.3% of caries-risk sites with GI and 8.3% of caries risk sites with GI-CPP-ACP (P>.05).
Conclusions: Both glass ionomers (GC Fuji II & GC Fuji VII) provided protection along the interface between the glass ionomer and cavosurface enamel, with the vast majority of caries risk sites. Majority of caries risk sites (87 to 91%) had no cavity wall lesion formation. Calcium phosphoeptide-amorphous calcium phosphate addition to glass ionomer (GC Fuji VII) resulted in significant reduction (P<.05) in caries lesion depth in enamel surfaces adjacent to the restorations, compared with glass inonomer without CPP-ACP (GC Fuji II). CPP-ACP incorporation into glass ionomer preventive and restorative materials may be beneficial in reducing caries development adjacent to sealed pit and fissures, and coronal and root surface restorations, especially in persons at risk for caries development. Note: Dental material products provided by GC America Corp.
Methods: 10 caries-free human molars underwent prophylaxis and cavity preparation in buccal and lingual surfaces. GI (GC Fuji II, GC Corp) and GI-CPP-ACP (GC Fuji VII, GC Corp) were placed in coronal buccal and lingual cavity preparations, per manufacturer's instructions. Acid-resistant varnish was applied, leaving 1mm windows of exposed surface enamel around restored cavities. Specimens were thermocycled in synthetic saliva for 24hrs. Artificial enamel caries were created (modified ten Cate method for 10d: alternating 8hrs demineralization followed by 16hrs remineralization). Longitudinal sections (3 sections/specimen, 60 lesions/group) were obtained. Cavity wall lesions presence and adjacent enamel surface lesion depths were determined using polarized light microscopy (water imbibition, ANOVA, DMR).
Results: Mean depths for adjacent enamel surface lesions were: GI Group: 352 ± 36um, and GI-CPP-ACP Group: 217 ± 23 um (P<.05). Enamel surface lesion depth reduction with GI-CPP-ACP restorations compared with GI restorations was 38% (P<.05). Wall lesions were confined to the superficial aspect of cavity walls, and present at 13.3% of caries-risk sites with GI and 8.3% of caries risk sites with GI-CPP-ACP (P>.05).
Conclusions: Both glass ionomers (GC Fuji II & GC Fuji VII) provided protection along the interface between the glass ionomer and cavosurface enamel, with the vast majority of caries risk sites. Majority of caries risk sites (87 to 91%) had no cavity wall lesion formation. Calcium phosphoeptide-amorphous calcium phosphate addition to glass ionomer (GC Fuji VII) resulted in significant reduction (P<.05) in caries lesion depth in enamel surfaces adjacent to the restorations, compared with glass inonomer without CPP-ACP (GC Fuji II). CPP-ACP incorporation into glass ionomer preventive and restorative materials may be beneficial in reducing caries development adjacent to sealed pit and fissures, and coronal and root surface restorations, especially in persons at risk for caries development. Note: Dental material products provided by GC America Corp.
