IADR Abstract Archives
Antimicrobial Effect of a Novel Denture Adhesive Containing S-PRG Filler
Objectives: In this study, we aimed to assess antimicrobial effect of a novel denture adhesive containing surface pre-reacted glass ionomer (S-PRG) filler on Candida albicans.
Methods: We prepared six different grades of denture adhesives containing S-PRG filler. Two particle size and three different amount of S-PRG filler were investigated: 1 µm-5wt%; 1 µm-7.5wt%; 1 µm-10wt%; 3 µm-5wt%; 3 µm-7.5wt%; 3 µm-10wt%. A denture adhesive without S-PRG filler was kept as control. C albicans ATCC18804 was inoculated into the Sabouraud glucose (SG) agar plate. Specimens of denture adhesives were contacted to the agar plate through a filter (MILLPORE, 1.0 µm) and were incubated at 37°C for 24 hours (n=6). After incubation, specimens were removed with the filter. Next, we sectioned agars immediately underneath the denture adhesives to three pieces and measured their weight. Then we added 500 µm phosphate buffered salts (PBS) to the sectioned agars, which were crushed using a homogenizer (Funakoshi). Homogenized fungus liquid was diluted 100 times using PBS. Obtained fungus liquid was smeared on SG agar plate, and then incubated at 37°C for 24 hours. After incubation, we counted colony forming unit (CFU) for each specimen. The number of CFU was statistically compared using Kruskal-Wallis test followed by multiple comparison using Dunn’s test (α=0.05).
Results: Two denture adhesives containing S-PRG filler (1µm-10wt% and 3µm-10wt%) significantly suppressed growth of C albicans as compared to the control denture adhesive. Smaller particle size of S-PRG filler (1 µm) tends to show higher effect than larger particle size (3 µm). Regarding S-PRG filler content, 10 wt% of S-PRG filler seems to be promising in terms of suppressed growth of C albicans.
Conclusions: In summary, 1 µm-10wt% or 3 µm-10wt% denture adhesives containing S-PRG filler showed antimicrobial effect to C albicans.
Methods: We prepared six different grades of denture adhesives containing S-PRG filler. Two particle size and three different amount of S-PRG filler were investigated: 1 µm-5wt%; 1 µm-7.5wt%; 1 µm-10wt%; 3 µm-5wt%; 3 µm-7.5wt%; 3 µm-10wt%. A denture adhesive without S-PRG filler was kept as control. C albicans ATCC18804 was inoculated into the Sabouraud glucose (SG) agar plate. Specimens of denture adhesives were contacted to the agar plate through a filter (MILLPORE, 1.0 µm) and were incubated at 37°C for 24 hours (n=6). After incubation, specimens were removed with the filter. Next, we sectioned agars immediately underneath the denture adhesives to three pieces and measured their weight. Then we added 500 µm phosphate buffered salts (PBS) to the sectioned agars, which were crushed using a homogenizer (Funakoshi). Homogenized fungus liquid was diluted 100 times using PBS. Obtained fungus liquid was smeared on SG agar plate, and then incubated at 37°C for 24 hours. After incubation, we counted colony forming unit (CFU) for each specimen. The number of CFU was statistically compared using Kruskal-Wallis test followed by multiple comparison using Dunn’s test (α=0.05).
Results: Two denture adhesives containing S-PRG filler (1µm-10wt% and 3µm-10wt%) significantly suppressed growth of C albicans as compared to the control denture adhesive. Smaller particle size of S-PRG filler (1 µm) tends to show higher effect than larger particle size (3 µm). Regarding S-PRG filler content, 10 wt% of S-PRG filler seems to be promising in terms of suppressed growth of C albicans.
Conclusions: In summary, 1 µm-10wt% or 3 µm-10wt% denture adhesives containing S-PRG filler showed antimicrobial effect to C albicans.
