IADR Abstract Archives
Effect of E-cigarette Exposure on the Colour of Resin-based Composite
Objectives: It is well established that tobacco cigarette smoke causes staining of resin-based composites (RBC). The objective of this work was to establish whether e-cigarettes caused colour change of RBC.
Methods: Disc-shaped specimens were made from RBC (shade A2, GrandioSO), polymerised for 20s (per surface) using an LED curing-unit (520 mW/cm2). Specimens were exposed for 5 consecutive days to air, cigarette smoke (3R4F research cigarette, University of Kentucky) or e-cigarette aerosol with either 0 mg/ml or 18 mg/ml nicotine strengths (Vype eTank Pro, Dark Cherry flavour). Specimens were stored dry at room temperature. A research smoking/vaping machine (Gram Research) followed standard protocols for smoke/aerosol generation (110 puffs, 55ml puff volume, 2 [smoke and air] or 3 [e-cigarette] second puff duration, 30 second interval, rectangular profile). Colour was measured via the CIE system using a spectrophotometer (Ci6XS, Xrite). Specimens were measured at baseline and then daily following exposure. A sample size calculation determined 6 specimens were required per arm (18 mg/ml e-cigarette verses air). Colour change (ΔE) was calculated each day and data were analysed using one-way ANOVA with Tukey’s post-hoc test. Normality was confirmed by the Shapiro-Wilk Test.
Results: After 5 days of exposure, mean ΔE values were 33.9, 3.1, 1.9, 1.9 for cigarette smoke, e-cigarette 18 mg/ml, e-cigarette 0 mg/ml and air, respectively. Cigarette smoke exposure produced a significantly higher colour change in RBC when compared to air, 18mg/ml e-cigarette or 0mg/ml e-cigarette (P<0.001). E-cigarette aerosol exposure (5 days), irrespective of nicotine concentrations, induced minimal colour changes and mean ΔE (or Δa, Δb, ΔL) values were comparable to the air control group (P>0.05).
Conclusions: E-cigarette aerosol exposure did not lead to significant colour change of RBC after 5 days of exposure. Future research should consider other variables (e-liquid flavour) and over longer durations.
Methods: Disc-shaped specimens were made from RBC (shade A2, GrandioSO), polymerised for 20s (per surface) using an LED curing-unit (520 mW/cm2). Specimens were exposed for 5 consecutive days to air, cigarette smoke (3R4F research cigarette, University of Kentucky) or e-cigarette aerosol with either 0 mg/ml or 18 mg/ml nicotine strengths (Vype eTank Pro, Dark Cherry flavour). Specimens were stored dry at room temperature. A research smoking/vaping machine (Gram Research) followed standard protocols for smoke/aerosol generation (110 puffs, 55ml puff volume, 2 [smoke and air] or 3 [e-cigarette] second puff duration, 30 second interval, rectangular profile). Colour was measured via the CIE system using a spectrophotometer (Ci6XS, Xrite). Specimens were measured at baseline and then daily following exposure. A sample size calculation determined 6 specimens were required per arm (18 mg/ml e-cigarette verses air). Colour change (ΔE) was calculated each day and data were analysed using one-way ANOVA with Tukey’s post-hoc test. Normality was confirmed by the Shapiro-Wilk Test.
Results: After 5 days of exposure, mean ΔE values were 33.9, 3.1, 1.9, 1.9 for cigarette smoke, e-cigarette 18 mg/ml, e-cigarette 0 mg/ml and air, respectively. Cigarette smoke exposure produced a significantly higher colour change in RBC when compared to air, 18mg/ml e-cigarette or 0mg/ml e-cigarette (P<0.001). E-cigarette aerosol exposure (5 days), irrespective of nicotine concentrations, induced minimal colour changes and mean ΔE (or Δa, Δb, ΔL) values were comparable to the air control group (P>0.05).
Conclusions: E-cigarette aerosol exposure did not lead to significant colour change of RBC after 5 days of exposure. Future research should consider other variables (e-liquid flavour) and over longer durations.