IADR Abstract Archives
Cotinus coggygria, a Promising Plant for a New Mouthwash
Objectives: In Dentistry, oral mouthwashes can be used in the recovery period after surgical procedures, in acute oral mucosal and gingival infections, when the plaque control of the individual is insufficient and as a support for mechanical cleaning applications. Cotinus coggygria is a traditionally used medicinal plant rich in flavonoid and phenolic compounds having antioxidant and anti-inflammatory properties. There are also studies in the literature revealing antioxidant, anticancer, antimicrobial, antiviral, anti-inflammatory and liver protective effects of C. coggygria. Considering the beneficial effects of C. coggygria, determining its possible toxic effects and whether it can be used in the clinic is quite important. The aim of our study was to determine the non-toxic concentrations of the leaf extract prior to preparing the mouthwash formulation in order to test the wound healing potential of the mouthwash.
Methods: We intended to evaluate the in vitro toxicity and wound healing effect of the C. coggygria leaf extract on HGF-1 human gingival fibroblast cell line. Cytotoxicity was evaluated with the methylthiazolyldiphenyl-tetrazoliumbromide (MTT) assay and ATP Bioluminescence assay. The wound healing effect was evaluated with the in vitro scratch assay. Cytotoxicity was measured in the concentrations between 0-1000 µg/ml.
Results: According to our MTT test results cell proliferation was accelerated. The ATP content was inhibited in a dose dependent manner and we found IC50>100 µg/ml. This IC50 value is more than five times higher than the concentration which is stated as the cytotoxic limit by the National Cancer Institute (IC50<20 µg/ml). Below the concentration 100 μg/ml the cell viability was found higher than 75% to 85%.
Conclusions: According to the results of our study, the leaf extract of C. coggygria does not have cytotoxic effects and accelerates cell proliferation potentially contributing to wound healing. These results confirm the plants traditional use and are promising regarding their use in clinical practice.
Methods: We intended to evaluate the in vitro toxicity and wound healing effect of the C. coggygria leaf extract on HGF-1 human gingival fibroblast cell line. Cytotoxicity was evaluated with the methylthiazolyldiphenyl-tetrazoliumbromide (MTT) assay and ATP Bioluminescence assay. The wound healing effect was evaluated with the in vitro scratch assay. Cytotoxicity was measured in the concentrations between 0-1000 µg/ml.
Results: According to our MTT test results cell proliferation was accelerated. The ATP content was inhibited in a dose dependent manner and we found IC50>100 µg/ml. This IC50 value is more than five times higher than the concentration which is stated as the cytotoxic limit by the National Cancer Institute (IC50<20 µg/ml). Below the concentration 100 μg/ml the cell viability was found higher than 75% to 85%.
Conclusions: According to the results of our study, the leaf extract of C. coggygria does not have cytotoxic effects and accelerates cell proliferation potentially contributing to wound healing. These results confirm the plants traditional use and are promising regarding their use in clinical practice.