IADR Abstract Archives
Mechanical Characterization of Silk Incorporated Dental Materials
Objectives: This study aimed to evaluate mechanical properties of silk reinforced glass-ionomer and resin dental restoratives via diametral tensile strength (DTS) and Vicker's hardness.
Methods: Silk cocoons from the species Bombyx mori were processed to produce an aqueous solution of silk fibroin which was subsequently lyophilized and pulverized into a powder. Silk fibroin powder was incorporated into a glass-ionomer (Ketac™Cem, 3M, St. Paul, MN) and a resin-based material (Beautifil-II, Shofu, Kyoto, Japan) in varying volumetric percentages (0%, 5%, 10%, and 15%). Samples were prepared into 6x3 (mm) disks for each volumetric group (n=21). Vicker’s hardness testing was conducted using the Micromet® 2104 (Buehler, Lake Bluff, IL), where 5 samples were randomly chosen from each group. Hardness values (HN) reflect the average of 3 surface readings per sample. DTS was evaluated using the Instron 5566A (Norwood, MA). DTS was determined by DTS=2P/πDt (P=load failure, D=sample diameter, and t=sample thickness). Mean HN and DTS values were obtained then compared using one-way ANOVA. Post Hoc analysis via Tukey’s test provided p-values for mean comparison.
Results: The DTS of the glass-ionomer was not significantly affected from 0% to 15% silk content. However, HN increased from 59.07 to 100.27 from 0% to 15%. In resin-containing samples, silk addition led to a slight decrease in DTS. Mean comparison between 0% and all silk-containing samples show statistically significant differences. Similarly, HN was reduced from 0% to 15 %. See TABLE below for details.
Conclusions: This study was the first of its kind to incorporate silk fibroin into conventional dental restoratives and evaluate their mechanical properties. Our results demonstrate the viability of incorporating silk into dental restoratives. The investigation of using silk as a component of a liner or bonding agent may be a particular point of interest for future research.
Methods: Silk cocoons from the species Bombyx mori were processed to produce an aqueous solution of silk fibroin which was subsequently lyophilized and pulverized into a powder. Silk fibroin powder was incorporated into a glass-ionomer (Ketac™Cem, 3M, St. Paul, MN) and a resin-based material (Beautifil-II, Shofu, Kyoto, Japan) in varying volumetric percentages (0%, 5%, 10%, and 15%). Samples were prepared into 6x3 (mm) disks for each volumetric group (n=21). Vicker’s hardness testing was conducted using the Micromet® 2104 (Buehler, Lake Bluff, IL), where 5 samples were randomly chosen from each group. Hardness values (HN) reflect the average of 3 surface readings per sample. DTS was evaluated using the Instron 5566A (Norwood, MA). DTS was determined by DTS=2P/πDt (P=load failure, D=sample diameter, and t=sample thickness). Mean HN and DTS values were obtained then compared using one-way ANOVA. Post Hoc analysis via Tukey’s test provided p-values for mean comparison.
Results: The DTS of the glass-ionomer was not significantly affected from 0% to 15% silk content. However, HN increased from 59.07 to 100.27 from 0% to 15%. In resin-containing samples, silk addition led to a slight decrease in DTS. Mean comparison between 0% and all silk-containing samples show statistically significant differences. Similarly, HN was reduced from 0% to 15 %. See TABLE below for details.
Conclusions: This study was the first of its kind to incorporate silk fibroin into conventional dental restoratives and evaluate their mechanical properties. Our results demonstrate the viability of incorporating silk into dental restoratives. The investigation of using silk as a component of a liner or bonding agent may be a particular point of interest for future research.
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