IADR Abstract Archives
Viscoelastic Properties of Fibrinogen-Based Gel for Dental Pulp Regeneration
Objectives: The aim of this study was to evaluate the viscoelastic properties of an injectable fibrinogen-based gel and of human dental pulp.
Methods: Fibrinogen-based gel was obtained by adding fresh human blood to a fibrinogen solution(lyophilized powder of bovine fibrinogen diluted in DMEM culture medium) at 37°C. Two groups were established to evaluate changes in viscoelastic properties of the gel depending on its blood and fibrinogen content. In the first group blood concentration varied from 0.05% to 0.08% while fibrinogen concentration remained at 3mg/ml. For the second group blood concentration was maintained(0.05%) while fibrinogen content varied(3-6mg/ml). Human dental pulp from freshly extracted premolars was used as positive control. Elastic modulus, linear viscoelastic regime and gelation time were determined via Atomic Force Microscopy(AFM) and rheological techniques. AFM measurements were performed with a 5 µm diameter-size spherical tips in liquid using 10nN trigger force. Linear viscoelastic functions and gelation time were characterized using small-amplitude oscillatory shear. Data passed normality tests and were analyzed by one-way ANOVA(p≤0.05).
Results: Mean elasticity for human dental pulp was 246 Pa (coronal pulp) and 645.75 Pa (root pulp).Gel elasticity for first group increased from 132.75 Pa to 681 Pa with blood concentration from 0.04% to 0.08% respectively. The increasement was mild from 0.04 % to 0.06 % and statistically significant(p <0,001) from 0.06% to 0.08%. Regarding the second group (0.05% blood and 3-6mg/ml fibrinogen) values varied from 132.75 Pa to 240.75 Pa, respectively. Elasticity of the gel increases with a statistically significance(p <0,001) while increasing fibrinogen concentration at a proportional rate. Rheological measurements corroborate AFM results and provide information about the long term stability of the gel.
Conclusions: Obtained fibrinogen-based gel can easily be custom to mimics dental pulp viscoelastic properties and to favor cell differentiation during pulp regeneration procedures.
Methods: Fibrinogen-based gel was obtained by adding fresh human blood to a fibrinogen solution(lyophilized powder of bovine fibrinogen diluted in DMEM culture medium) at 37°C. Two groups were established to evaluate changes in viscoelastic properties of the gel depending on its blood and fibrinogen content. In the first group blood concentration varied from 0.05% to 0.08% while fibrinogen concentration remained at 3mg/ml. For the second group blood concentration was maintained(0.05%) while fibrinogen content varied(3-6mg/ml). Human dental pulp from freshly extracted premolars was used as positive control. Elastic modulus, linear viscoelastic regime and gelation time were determined via Atomic Force Microscopy(AFM) and rheological techniques. AFM measurements were performed with a 5 µm diameter-size spherical tips in liquid using 10nN trigger force. Linear viscoelastic functions and gelation time were characterized using small-amplitude oscillatory shear. Data passed normality tests and were analyzed by one-way ANOVA(p≤0.05).
Results: Mean elasticity for human dental pulp was 246 Pa (coronal pulp) and 645.75 Pa (root pulp).Gel elasticity for first group increased from 132.75 Pa to 681 Pa with blood concentration from 0.04% to 0.08% respectively. The increasement was mild from 0.04 % to 0.06 % and statistically significant(p <0,001) from 0.06% to 0.08%. Regarding the second group (0.05% blood and 3-6mg/ml fibrinogen) values varied from 132.75 Pa to 240.75 Pa, respectively. Elasticity of the gel increases with a statistically significance(p <0,001) while increasing fibrinogen concentration at a proportional rate. Rheological measurements corroborate AFM results and provide information about the long term stability of the gel.
Conclusions: Obtained fibrinogen-based gel can easily be custom to mimics dental pulp viscoelastic properties and to favor cell differentiation during pulp regeneration procedures.