IADR Abstract Archives
Histopathological Evaluation of BCP-cement on Rat Calvarial Model
Objectives: Biphasic calcium phosphate (BCP) cement, consisting of an α-tricalcium phosphate-tetracalcium phosphate as the only solid phase and a calcium phosphate solution (Ca-P soln) as the liquid phase, was recently reported. The BCP-cement provided sufficient physical properties for clinical uses. Objective of this study was to evaluate the new bone (NB) formation of BCP-cement when grafted on exposed rat calvaria using plastic caps.
Methods: BCP-cement was prepared by mixing BCP powder with Ca-P soln at P/L of 3.0. A commercially available cements (Biopex-R, BoneSource, OsteoVation) were used as controls. A circular groove was made on calvaria with an inner diameter of 5mm and small holes, without penetration to the dura, were drilled to induce bleeding from the bone marrow. A cylindrical plastic cap, filled with each material, was pressed into the circular grooves. At 0 and 8 weeks after surgery, X-ray micro-CT image for each surgical site was taken. Fluorescent labeling agents were injected into abdomen at the 1 and 3 weeks before sacrifice. 8 weeks after the surgery, the animals were sacrificed, and biopsies were obtained and separated into 2 parts. Half of the undecalcified sections were subjected to fluorescent labelling analysis (FLA) for NB area, and the other half of the decalcified sections were subjected to HE and TB staining for histopathological examinations. Computer-assisted histometric measurements of NB area, which identified by FLA, were performed using NIH image analysis. Bone conversion (BC) rate was determined by measuring the NB area in filling area, and was expressed as a percentage of original filling area.
Results: The BC rate of the BCP-cement (9.21±1.33 %) exhibited apparently significantly larger (p<0.05) than that of controls (Biopex-R:3.91±2.30 %, BoneSource:4.52±2.34 %, OsteoVation:2.95±1.36 %). These results were confirmed by decalcified staining sections and micro-CT observations.
Conclusions: The results indicated that the BCP-cement had osteoconductive potential for bone augmentations.
Methods: BCP-cement was prepared by mixing BCP powder with Ca-P soln at P/L of 3.0. A commercially available cements (Biopex-R, BoneSource, OsteoVation) were used as controls. A circular groove was made on calvaria with an inner diameter of 5mm and small holes, without penetration to the dura, were drilled to induce bleeding from the bone marrow. A cylindrical plastic cap, filled with each material, was pressed into the circular grooves. At 0 and 8 weeks after surgery, X-ray micro-CT image for each surgical site was taken. Fluorescent labeling agents were injected into abdomen at the 1 and 3 weeks before sacrifice. 8 weeks after the surgery, the animals were sacrificed, and biopsies were obtained and separated into 2 parts. Half of the undecalcified sections were subjected to fluorescent labelling analysis (FLA) for NB area, and the other half of the decalcified sections were subjected to HE and TB staining for histopathological examinations. Computer-assisted histometric measurements of NB area, which identified by FLA, were performed using NIH image analysis. Bone conversion (BC) rate was determined by measuring the NB area in filling area, and was expressed as a percentage of original filling area.
Results: The BC rate of the BCP-cement (9.21±1.33 %) exhibited apparently significantly larger (p<0.05) than that of controls (Biopex-R:3.91±2.30 %, BoneSource:4.52±2.34 %, OsteoVation:2.95±1.36 %). These results were confirmed by decalcified staining sections and micro-CT observations.
Conclusions: The results indicated that the BCP-cement had osteoconductive potential for bone augmentations.
