A Novel Model System to Study the Matrix-Mediated Attachment of Epithelial Cells to Mineralized Tooth Surfaces

Objectives: We propose to develop a model system to study the epithelial attachment/detachment between the tooth and the JE with an emphasis on the combined role of LAM332 and SCPPPQ1.
Methods: Recombinant human SCPPPQ1 and LAM332 were used, alone or in combination. Epithelium-derived human squamous cell carcinoma (HSC-2) cells were used as a model of nonkeratinized oral epithelial cells. Sintered hydroxyapatite (HA) discs were used as a bioceramic analogue to the mineral component of the enamel surface. Solid surfaces were coated with different mixtures of LAM332-SCPPPQ1 and incubated with cells. The kinetics of attachment/detachment and the morphology of the cells on different substrates were studied using live cell imaging, fluorescent microscopy, microfluidic assays, and scanning electron microscopy (SEM).
Results: The adhesion strength of the cells at certain molar ratio of SCPPPQ1 to LAM332 is higher comparing with LAM332 or SCPPPQ1 alone. Cell attachment to the SCPPPQ1/LAM332 complex was much faster and stronger compared to controls.
Conclusions: We successfully developed an in vitro model system that enables us to investigate the role of enamel proteins in the epithelial attachment to mineralized tooth surfaces. Application of the LAM332/SCPPPQ1 complex may become an option to enhance the attachment of epithelial tissues to the tooth and possibly implant surfaces in a clinical setting.