IADR Abstract Archives
Antimicrobial Activity and Optical Properties of Experimental Resin Containing Ag@SiO2NPs
Objectives: To verify the effect of silica-coated silver nanoparticles (Ag@SiO2NPs) on Streptococcus mutans biofilm formation, degree of conversion and optical properties of experimental resin-based materials.
Methods: Four experimental materials were prepared containing a BisGMA/TEGDMA matrix and 1, 3 or 5wt% of Ag@SiO2NPs, as well as a negative control group (resin without NPs). The antimicrobial activity of the experimental materials against Streptococcus mutans was assessed according to the numbers of colony-forming units (CFU, n=3). Degree of conversion (DC, n=3) after 24h was determined by FTIR spectroscopy. Optical properties (delta E and transmittance) were evaluated using spectrophotometry (n=12). Data were analyzed by one-way ANOVA/Tukey test (alpha:5%).
Results: The antimicrobial analysis presented a statistically significant difference for the groups containing Ag@SiO2NPs compared to the control group (p<0.001). The UFCs counts of up to 87% lower (5% = 8.7 x 106; 3%= 2 x 107; 1% = 1.8 x 107) than the control group (6.5 x 107), however without statistical difference among the mass of Ag@SiO2NPs. The four experimental groups showed similar DC values, ranging between 84 and 85% (p>0.05). All groups presented significant difference among them in optical analysis and the increase in NP content significantly affected transmittance and delta E values. Transmittance percentage at 470nm was lower in materials with Ag@SiO2NPs (5% = 23%; 3% = 35%; 1% = 56%) in comparison to the control (67%, p<0.001). The group that contains 5% of Ag@SiO2NPs showed delta E value (28) about five times higher than the value observed in the group with 1% of Ag@SiO2 NPs (5), and group 3% showed intermediate value (17, p<0.001).
Conclusions: The addition of 1, 3 and 5wt% of Ag@SiO2 NP in the resin matrix was effective in reducing Streptococcus mutansbiofilm growth. DC was not affected, however, NP significantly affected the shade and transmittance of experimental materials.
Methods: Four experimental materials were prepared containing a BisGMA/TEGDMA matrix and 1, 3 or 5wt% of Ag@SiO2NPs, as well as a negative control group (resin without NPs). The antimicrobial activity of the experimental materials against Streptococcus mutans was assessed according to the numbers of colony-forming units (CFU, n=3). Degree of conversion (DC, n=3) after 24h was determined by FTIR spectroscopy. Optical properties (delta E and transmittance) were evaluated using spectrophotometry (n=12). Data were analyzed by one-way ANOVA/Tukey test (alpha:5%).
Results: The antimicrobial analysis presented a statistically significant difference for the groups containing Ag@SiO2NPs compared to the control group (p<0.001). The UFCs counts of up to 87% lower (5% = 8.7 x 106; 3%= 2 x 107; 1% = 1.8 x 107) than the control group (6.5 x 107), however without statistical difference among the mass of Ag@SiO2NPs. The four experimental groups showed similar DC values, ranging between 84 and 85% (p>0.05). All groups presented significant difference among them in optical analysis and the increase in NP content significantly affected transmittance and delta E values. Transmittance percentage at 470nm was lower in materials with Ag@SiO2NPs (5% = 23%; 3% = 35%; 1% = 56%) in comparison to the control (67%, p<0.001). The group that contains 5% of Ag@SiO2NPs showed delta E value (28) about five times higher than the value observed in the group with 1% of Ag@SiO2 NPs (5), and group 3% showed intermediate value (17, p<0.001).
Conclusions: The addition of 1, 3 and 5wt% of Ag@SiO2 NP in the resin matrix was effective in reducing Streptococcus mutansbiofilm growth. DC was not affected, however, NP significantly affected the shade and transmittance of experimental materials.
