IADR Abstract Archives
Activity of Tetracycline-HCl diluted in Three Vehicles on Periodontopathogens
Objectives: Background: The development and progression of periodontal diseases results in the dynamic interaction of microorganisms in their habitat and their alteration generates a dysbiotic state. Fusobacterium nucleatum and Prevotella intermedia are microorganisms that work as bridges between the pioneer communities and microorganisms of the red complex responsible for periodontitis such as Porphyromonas gingivalis. The use of tetracycline-HCl as an adjunct to periodontal treatment is commonly used as an antiseptic but there are no clear dilution or concentration protocols. It is discussed whether the action is by pH or by tetracycline.
The aim of this work was to evaluate the antimicrobial activity of tetracycline-HCl, dissolved in three vehicles: distilled water, saline solution and lidocaine-2% with epinephrine-1:80,000 in acid and basic pH in cultures of Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis.
Methods: The antimicrobial activity of tetracycline-HCl was evaluated at pH-1.8 and pH-7 in concentration of 125, 250 and 500mg dissolved in distilled water, saline solution and lidocaine-2% with epinephrine-1:80,000; at different times of action: 30, 60 and 120 seconds on Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis. The Kelsey Maurer technique was used verifying the presence or absence of colony forming units, each trial was performed in triplicate with its viability controls. Descriptive statistics are performed, and as a summary measure, percentages were used.
Results: Inhibition of Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis by tetracycline-HCl is showed in concentrations of 125, 250 and 500mg dissolved in distilled water, saline solution and lidocaine-2% with epinephrine-1:80,000, at times of 30, 60 and 120 seconds in acidic and neutral pH.
Conclusions: The results that affect tetracycline-HCl constitute a good antimicrobial alternative like an antiseptic for Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis indifferent to the pH, the vehicle in which it was the delivery, the concentration taken and the times used here.
The aim of this work was to evaluate the antimicrobial activity of tetracycline-HCl, dissolved in three vehicles: distilled water, saline solution and lidocaine-2% with epinephrine-1:80,000 in acid and basic pH in cultures of Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis.
Methods: The antimicrobial activity of tetracycline-HCl was evaluated at pH-1.8 and pH-7 in concentration of 125, 250 and 500mg dissolved in distilled water, saline solution and lidocaine-2% with epinephrine-1:80,000; at different times of action: 30, 60 and 120 seconds on Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis. The Kelsey Maurer technique was used verifying the presence or absence of colony forming units, each trial was performed in triplicate with its viability controls. Descriptive statistics are performed, and as a summary measure, percentages were used.
Results: Inhibition of Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis by tetracycline-HCl is showed in concentrations of 125, 250 and 500mg dissolved in distilled water, saline solution and lidocaine-2% with epinephrine-1:80,000, at times of 30, 60 and 120 seconds in acidic and neutral pH.
Conclusions: The results that affect tetracycline-HCl constitute a good antimicrobial alternative like an antiseptic for Fusobacterium nucleatum, Prevotella intermedia and Porphyromonas gingivalis indifferent to the pH, the vehicle in which it was the delivery, the concentration taken and the times used here.
