IADR Abstract Archives
A Novel Biodegradable Strontium-modified Composite-hydrogel as Potential Bone Regeneration Biomaterial
Objectives: Prompt bone neoformation is of utmost importance in biomedical science and a challenge for current biomaterials. This study aimed to synthetize and analyze a new composite hydrogel containing hydroxyapatite and strontium-hydroxyapatite (HAp+Sr) particles as a potential bone regeneration biomaterial.
Methods: A biodegradable, eco-friendly crosslinked hydrogel was prepared using chondroitin-sulfate and polyvinyl-alcohol through cyclic freeze-thawing. HAp and HAp+Sr particles were synthesized by a wet precipitation and calcination method and added to the hydrogel at 5, 10, or 15wt%. The particles and hydrogels were characterized by scanning electron microscopy and X-ray dispersive energy spectroscopy (SEM-EDX). Hydrogel porosity was assessed by computerized microtomography (micro-CT). In vitro characterization of the composite hydrogel included total porosity, water sorption, swelling, compressive strength, and cell-viability.
Results: Synthesis of all composite hydrogels was achieved using high purity reagents with a simple and low-cost protocol that could be escalated for commercial purposes. Particles showed irregular shape in SEM, EDX confirmed a homogeneous distribution of Ca+ and Sr+ along particles. The regular distribution of HAp and HAp+Sr was observed irrespective of particle concentration added. Micro-CT analysis exhibited a 30% total porosity of the control hydrogel and a trend to reduce total porosity as the proportion of particles increased. Water sorption, porosity, and compressive strength of the composite hydrogel increased when 10wt% particles were added. Cell viability test revealed increased proliferation of Dental-Pulp-Stem-Cells (DPSC) in all groups including the control hydrogel (no particles).
Conclusions: In conclusion, a modified composite hydrogel with up to 15wt% HAp or HAp+Sr particles was successfully synthetized with improved physical-chemical properties as compared with the control hydrogel, also showing promising manipulative characteristics. The increased cell proliferation indicates that this novel composite hydrogel could have a positive role as biomedical material. Next steps will include in-vivo testing for bone regeneration in animal model.
Methods: A biodegradable, eco-friendly crosslinked hydrogel was prepared using chondroitin-sulfate and polyvinyl-alcohol through cyclic freeze-thawing. HAp and HAp+Sr particles were synthesized by a wet precipitation and calcination method and added to the hydrogel at 5, 10, or 15wt%. The particles and hydrogels were characterized by scanning electron microscopy and X-ray dispersive energy spectroscopy (SEM-EDX). Hydrogel porosity was assessed by computerized microtomography (micro-CT). In vitro characterization of the composite hydrogel included total porosity, water sorption, swelling, compressive strength, and cell-viability.
Results: Synthesis of all composite hydrogels was achieved using high purity reagents with a simple and low-cost protocol that could be escalated for commercial purposes. Particles showed irregular shape in SEM, EDX confirmed a homogeneous distribution of Ca+ and Sr+ along particles. The regular distribution of HAp and HAp+Sr was observed irrespective of particle concentration added. Micro-CT analysis exhibited a 30% total porosity of the control hydrogel and a trend to reduce total porosity as the proportion of particles increased. Water sorption, porosity, and compressive strength of the composite hydrogel increased when 10wt% particles were added. Cell viability test revealed increased proliferation of Dental-Pulp-Stem-Cells (DPSC) in all groups including the control hydrogel (no particles).
Conclusions: In conclusion, a modified composite hydrogel with up to 15wt% HAp or HAp+Sr particles was successfully synthetized with improved physical-chemical properties as compared with the control hydrogel, also showing promising manipulative characteristics. The increased cell proliferation indicates that this novel composite hydrogel could have a positive role as biomedical material. Next steps will include in-vivo testing for bone regeneration in animal model.
