Anticancer Effect of Resveratrol on Squamous Cell Carcinoma
Objectives: To investigate the anticancer effect of resveratrol on head and neck squamous cell carcinoma (HNSCC) both ex-vivo (HEp-2 cell line) and in-vivo (experimental model). Methods: In silico data analysis was used to retrieve genes linked to autophagy and HNSCC. Docking analysis between resveratrol and the chosen genes was then performed. MTT assay was used to investigate the cytotoxic effect of IC50 of resveratrol on HEp-2 cell line after 48hours. Quantitative real time PCR was performed to detect the level changes in the genes. Rat model was used to validate the results in combination with 5-Florouracyl (5-FU). Results: The linked genes to autophagy and HNC were miRNA-20a and SQSTM1 retrieved from mirWalk and Gene atlas expression database respectively. Docking showed that resveratrol interacted with miRNA-20a and less affinity towards SQSTM1 showing free binding energy of -22.51 and -20.24 Kcal/mol. The mean viability % of IC50 for 48 hours was 46.7% and the mean fold change for miRNA-20a and SQSTM1 was 480.6 and 505.7 respectively. The clinical evaluation of the albino rats treated with a combination of resveratrol and 5-FU showed better recovery and the least necrotic tongue debris. Histopathological and immuohistochemical evaluation of this group showed mild dysplasia, and mild mutant p53 immunoreactivity compared to moderate-severe dysplastic changes and micro-invasions observed in other groups.
Conclusions: Resveratrol has a promising anticancer effect against HNSCC
Division:IADR/AADR/CADR General Session
Meeting:2020 IADR/AADR/CADR General Session (Washington, D.C., USA) Location:Washington, D.C., USA
Year: 2020 Final Presentation ID:1998 Abstract Category|Abstract Category(s):Oral Medicine & Pathology Research
Authors
El Shafei, Marwa
( Misr International University
, Cairo
, Egypt
)