Caries Preventing Effectiveness of New Isomalt-containing Mouthrinse Formulations

Objectives: This study investigated the effectiveness of Isomalt-containing mouthrinse to prevent enamel demineralization, compared with sodium fluoride mouthrinse (225 ppm fluoride).
Methods: 150 tooth blocks were randomly assigned to five experimental groups (30 blocks/group): De-ionized distilled water (A), and mouthrinse containing either 1% Isomalt with 225ppm fluoride and 0.05% cetylpyridinium chloride (CPC), (B), 1% Isomalt with 225ppm fluoride (C), 225 ppm fluoride with 0.05% CPC (D), or 225 ppm fluoride (E). The five groups were subjected to 7-day demineralization by plaque growth in a multispecies Microbial Caries Model. Mouthrinses were applied once daily for 1 minute. Demineralization was assessed using Surface Microhardness testing for change in SMH (△SMH) and Transverse Microradiography (TMR) for mineral loss (△Z). All pairwise contrasts were tested using paired t-test (Intra-group comparison in SMH) and ANOVA followed by Tukey’s for intergroup comparisons in △SMH and △Z. All p-value are considered significant if <0.05.
Results: Relative to sound enamel baseline, paired t-test indicated significant (P<0.001) decrease in SMH (demineralization) in groups A, C, D and E but not in group B. Intergroup comparison using percentage change in SMH (%△SMH) showed significantly (Tukey’s; p<0.001) greater demineralization in group A compared to all treatment groups; however, there was a significantly (P<0.001) greater demineralization in groups C, D and E compared to B. No difference among groups C, D and E. For TMR data, relative to group A, all groups significantly (p<0.0001) inhibited demineralization at varying percentages; however, all comparisons of the groups with each other were statistically significant (p<0.0001), except group C vs. E.
Conclusions: The present study demonstrated the effectiveness of a mouthrinse containing 1% Isomalt with 225ppm fluoride and 0.05% CPC in inhibiting tooth demineralization in the presence bacterial plaque; thus highlighting its potential as a more effective caries control tool than mouthrinse with 225ppm fluoride with/without CPC.