X-Ray Nano-particulate Markers Application  for Visualization of Dentine-Restorative Material Interfaces

This study aims to develop the technique of tooth sample preparation allowing for visualization of intermediate layers and interfaces between dentine and restorative materials and estimate the limits of applicability of adhesive systems in root canals.

Method:

A SEM Mira//LMU, “TESCAN” was selected to produce  high magnification images of surface morphology of tooth hard tissues with the ability to generate localized chemical information using an EDX system Inca Energy 350, “Oxford Instruments”. To visualize intermediate layers between dentine and restoration X-ray nano-particulate  markers were embedded into adhesive system to give contrast between the adhesive, tooth substances and restorative materials.

Result:

The powder of silver nanoparticles was selected in the present study. To achieve  homogenious distribution of nanoparticles  in adhesive  an ultrasound set HD2070 was used. The  tooth specimens were prepared allowing for observation under the microscope.

The developed method of visualization of intermediate layers offers the possibility to study the penetration depth of adhesive systems of different generations in root dentine of post-core restored teeth.

The distribution of Ag in dentine structures confirms adhesive penetration in root dentine. Irregular detection of Ag could be the result of looping and twisting of dentinal tubules, nanoparticles conglomeration, inhomogeneous distribution of nanoparticles. The depth of adhesive penetration depends on the correlation between the directions of dentinal tubules, bonding resin delivery and gravity.

Conclusion:

In this study the methodology of biological sample preparation for dental research using SEM/EDX  has been elaborated. A novel adhesive system with embedded X-ray nano-particulate markers has been designed. The technique allowing for visualization of intermediate layers and interfaces between dentine and restorative materials using the X-ray nano-particulate markers has been developed and approved. These methods and approaches have been used to compare the objective depth of penetration of adhesive systems of different generations in root dentine.