Matrigel is a natural hydrogel with appropriate features for oral tissue regeneration using tissue engineering. The objective of this research was evaluate the biocompatibility of matrigel with mesenchymal stem cells from human gingival tissue.
Method:
Mesenchymal stem cells were isolated from human gingival connective tissue (GMSC), in the passage 4 immunophenotype was characterized by flow cytometry, using antibodies specific for mesenchymal stem cell lineage and were differentiated into adipocytes, osteoblasts and chondroblasts following the criteria established by the ISTC. A natural hydrogel scaffold was used (Matrigel, BDTM) and cultured with GMSC (10.000 cells/cm2) in alpha MEM supplemented with 10% FBS. To assess the biocompatibility of Matrigel BDTM developed an analysis of transport capacity, cell membrane integrity and evaluation of cell doubling time by recultivation of the matrix with MSCG, trypan blue staining and software doubling time respectively.
Result:
At seven days of reculture, the cells begin to migrate and proliferate to reach a confluence of 80% at 14 days. To evaluate cell membrane integrity was observed at 7 days viability of 70%. The doubling time showed an increase in cell proliferation.
Conclusion:
Matrigel proved to be biocompatible for gingival mesenchymal stem cells and could be appropriate in oral tissue regeneration using tissue engineering